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IL-1 alpha/IL-1A Protein, Human, Recombinant (His & Avi), Biotinylated

(Synonyms: Interleukin-1 α, Interleukin-1 Alpha, IL1F1, IL1A, IL-1 α, IL-1 Alpha, Hematopoietin-1) Copy Product Info

Synonyms: Interleukin-1 α, Interleukin-1 Alpha, IL1F1, IL1A, IL-1 α, IL-1 Alpha, Hematopoietin-1

Catalog No. TMPJ-00161 Copy Product Info
IL-1 alpha/IL-1A Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is P01583.
Pack SizePriceUSA StockGlobal StockQuantity
5 μg$1297-10 days7-10 days
10 μg$2137-10 days7-10 days
20 μg$3557-10 days7-10 days
50 μg$6257-10 days7-10 days
100 μg$9637-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Bioactivity
Human IL-1R1, No Tag immobilized on CM5 Chip can bind Biotinylated Human IL-1 alpha, His-Avi Tag with an affinity constant of 1.01 μM as determined in SPR assay (Biacore T200).
Description
IL-1 alpha/IL-1A Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is P01583.
Species
Human
Expression System
HEK293 Cells
TagC-His-Avi
Accession NumberP01583
Amino AcidSer113-Ala271
ConstructionSer113-Ala271
Protein Purity
> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
Endotoxin< 1 EU/µg as determined by LAL test.
FormulationLyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
ReconstitutionReconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & StorageLyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
SynonymsInterleukin-1 α, Interleukin-1 Alpha, IL1F1, IL1A, IL-1 α, IL-1 Alpha, Hematopoietin-1
Research Background
Interleukin-1 alpha (IL1α) is a cytokine member of the interleukin-1 family. IL-1 consists of two distinct forms: IL1α and IL1β that recognize the same cell surface receptors but are distinct proteins with approximately 25% amino acid sequence identity. IL1α is constitutively produced by epithelial cells and plays an essential role in maintenance of skin barrier function. Upon stimulation, a wide variety of cells including osteoblasts, monocytes, macrophages can be induced to express IL1α. IL1α possesses a wide range of metabolic, physiological, haematopoietic activities, and is critically involved in the regulation of the immune responses and inflammatory responses.
Chemical Properties
Molecular Weight20.95 kDa (predicted); 25-32 kDa (due to glycosylation)
Storage & Solubility Information
StorageLyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Related Tags: IL-1 alpha/IL-1A Protein, Human, Recombinant (His & Avi), Biotinylated chemical structure | IL-1 alpha/IL-1A Protein, Human, Recombinant (His & Avi), Biotinylated molecular weight