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IL-18 Protein, Human, Recombinant
(Synonyms: Interleukin 18, IL-1g, IL1F4, IL-18, IGIF) Copy Product InfoSynonyms: Interleukin 18, IL-1g, IL1F4, IL-18, IGIF
Catalog No. TMPY-00277 Copy Product Info
IL-18 Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 18.2 kDa and the accession number is Q14116-1.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $63 | 7-10 days | 7-10 days | |
| 10 μg | $100 | - | In Stock | |
| 20 μg | $159 | 7-10 days | 7-10 days | |
| 50 μg | $268 | 7-10 days | 7-10 days | |
| 100 μg | $397 | 7-10 days | 7-10 days | |
| 200 μg | $698 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
Batch Information
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Purity:SDS-PAGE: 95.4%; SEC-HPLC: 99.6%
Appearance:Lyophilized powder
Color:White
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | 1.Measured by its ability to induce IFN-gamma secretion by KG‑1 human acute myelogenous leukemia cells in the presence of TNF-alpha. The ED50 for this effect is typically 5-30 ng/ml.
2.Loaded Recombinant Human IL-18R beta / IL-18RAP Protein, Fc Tag on ProA Biosensor, can bind Recombinant Human IL-18Rα Protein, His Tag (Premixed with Human IL-18, Tag Free) with an affinity constant of 7.7nM as determined in BLI assay (Routinely tested).   |
| Description | IL-18 Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 18.2 kDa and the accession number is Q14116-1. |
| Species | Human |
| Expression System | E. coli |
| Tag | Tag Free |
| Accession Number | Q14116-1 |
| Construction | A DNA sequence encoding the human IL18 (NP_001553.1) (Tyr37-Asp193) was expressed. Predicted N terminal: Tyr 37 |
| Protein Purity | > 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.   |
| Endotoxin | < 10 EU/mg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstituted with sterile deionized water to 0.15 mg/mL. Reconstitution conditions may vary depending on the lot. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | Interleukin 18, IL-1g, IL1F4, IL-18, IGIF |
| Research Background |
| Molecular Weight | 18.2 kDa (predicted) |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: IL-18 Protein, Human, Recombinant chemical structure | IL-18 Protein, Human, Recombinant molecular weight
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