IL-18 Protein, Human, Recombinant

Name: IL-18 Protein, Human, Recombinant
Brand: TargetMol
SKU: TMPY-00277
Rating: 4.5 (1 reviews)

(Synonyms: Interleukin 18, IL-1g, IL1F4, IL-18, IGIF) Copy Product Info

Synonyms: Interleukin 18, IL-1g, IL1F4, IL-18, IGIF

Catalog No. TMPY-00277 Copy Product Info

IL-18 Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 18.2 kDa and the accession number is Q14116-1.

Pack Size	Price	USA Stock	Global Stock
5 µg	$63	7-10 days	7-10 days
10 µg	$100	-	In Stock
20 µg	$159	7-10 days	7-10 days
50 µg	$268	7-10 days	7-10 days
100 µg	$397	7-10 days	7-10 days
200 µg	$698	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:SDS-PAGE: 95.4%; SEC-HPLC: 99.6%

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	1.Measured by its ability to induce IFN-gamma secretion by KG‑1 human acute myelogenous leukemia cells in the presence of TNF-alpha. The ED50 for this effect is typically 5-30 ng/ml. 2.Loaded Recombinant Human IL-18R beta / IL-18RAP Protein, Fc Tag on ProA Biosensor, can bind Recombinant Human IL-18Rα Protein, His Tag (Premixed with Human IL-18, Tag Free) with an affinity constant of 7.7nM as determined in BLI assay (Routinely tested).
Description	IL-18 Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 18.2 kDa and the accession number is Q14116-1.
Species	Human
Expression System	E. coli
Tag	Tag Free
Accession Number	Q14116-1
Construction	A DNA sequence encoding the human IL18 (NP_001553.1) (Tyr37-Asp193) was expressed. Predicted N terminal: Tyr 37
Protein Purity	> 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC. ,
Endotoxin	< 10 EU/mg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.15 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	Interleukin 18, IL-1g, IL1F4, IL-18, IGIF
Research Background	Interleukin-18 (IL-18, also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of IL-18 and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. IL-18 is an IL-1-like cytokine that requires cleavage with caspase-1 to become active, was found to increase IgE production in a CD4+ T cell -, IL-4- and STAT6-dependent fashion. IL-18 and T cell receptor-mediated stimulation could induce naive CD4+ T cells to develop into IL-4-producing cells in vitro. Thus, caspase-1 and IL-18 may be critical in the regulation of IgE production in vivo, providing a potential therapeutic target for allergic disorders. IL-18 production in primary synovial cultures and purified synovial fibroblasts was, in turn, upregulated by TNF-α and IL-1β, suggesting that monokine expression can feedback to promote Th1 cell development in the synovial membrane. Besides, synergistic combinations of IL-18, IL-12, and IL-15 may be of importance in sustaining both Th1 responses and monokine production in RA.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

Chemical Properties

Molecular Weight

18.2 kDa (predicted)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.