IL-15 Protein, Human, Recombinant

The protein encoded by the IL15 gene is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and interleukine 2 share many biological activities. They are found to bind common hematopoietic receptor subunits and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between this cytokine and IL2. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. Studies of the mouse counterpart suggested that this cytokine may increase the expression of apoptosis inhibitor BCL2L1/BCL-x(L), possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. Alternatively, spliced transcript variants of this gene have been reported.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

Pack Size	Availability	Price/USD
10 μg	In stock	$ 126.00
20 μg	5 days	$ 192.00
50 μg	5 days	$ 329.00
100 μg	5 days	$ 498.00
200 μg	5 days	$ 821.00
500 μg	5 days	$ 2,470.00

Pack Size

Availability

Price/USD

Quantity

10 μg

In stock

$ 126.00

20 μg

5 days

$ 192.00

50 μg

5 days

$ 329.00

100 μg

5 days

$ 498.00

200 μg

5 days

$ 821.00

500 μg

5 days

$ 2,470.00

Biological Information

1. Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is typically 0.5-5 ng/mL. 2. Labeled biotin to IL-15 Protein, Human, Recombinant by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of IL-15 Protein, Human, Recombinant, Biotinylated bound to Anti-IL15 Antibody was 0.1nM. (Routinely tested)

Description

Species	Human
Expression System	E. coli
Tag	Tag Free
Accession Number	P40933-1
Synonyms	IL-15, interleukin 15
Construction	A DNA sequence encoding the human IL15 (NP_000576.1) (Asn49-Ser162) was expressed.
Protein Purity	≥ 95 % as determined by SDS-PAGE.
Molecular Weight	Approxiamtely 12.8 kDa

Endotoxin	< 20 EU per mg of the protein.
Formulation	Lyophilized from sterile 50 mM Tris, 200 mM NaCl, pH 8.0. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	The protein encoded by the IL15 gene is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and interleukine 2 share many biological activities. They are found to bind common hematopoietic receptor subunits and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between this cytokine and IL2. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. Studies of the mouse counterpart suggested that this cytokine may increase the expression of apoptosis inhibitor BCL2L1/BCL-x(L), possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. Alternatively, spliced transcript variants of this gene have been reported.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy