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IL-3 Protein, Mouse, Recombinant

(Synonyms: PSF, MCGF, interleukin 3, Il-3, HCGF, Csfmu, BPA) Copy Product Info

Synonyms: PSF, MCGF, interleukin 3, Il-3, HCGF, Csfmu, BPA

Catalog No. TMPY-04845 Copy Product Info
IL-3 Protein, Mouse, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 15.7 kDa and the accession number is P01586.

Batch Information

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Purity:92.7%
Appearance:Lyophilized powder
Color:White
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Product Introduction

Bioactivity
Bioactivity
Measured in a cell proliferation assay using NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 0.1-0.5 ng/mL.
Description
IL-3 Protein, Mouse, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 15.7 kDa and the accession number is P01586.
Species
Mouse
Expression System
HEK293 Cells
TagTag Free
Accession NumberP01586
ConstructionA DNA sequence encoding the mouse IL3 (NP_034686.2) (Met1-Cys166) was expressed. Predicted N terminal: Ala 27
Protein Purity
> 95 % as determined by SDS-PAGE.
IL-3 Protein, Mouse, Recombinant
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionA Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
SynonymsPSF, MCGF, interleukin 3, Il-3, HCGF, Csfmu, BPA
Research Background
IL3 (interleukin 3), also known as IL-3, is a potent growth-promoting cytokine that belongs to the IL-3 family. IL3/IL-3 also belongs to the group of interleukins. Interleukins are produced by a wide variety of body cells. The function of the immune system depends in a large part on interleukins, and rare deficiencies of a number of them have been described, all featuring autoimmune diseases or immune deficiency. The majority of interleukins are synthesized by helper CD4+ T lymphocytes, as well as through monocytes, macrophages, and endothelial cells. They promote the development and differentiation of T, B, and hematopoietic cells. IL3/IL-3 is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation, and apoptosis. IL3/IL-3 has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.
Chemical Properties
Molecular Weight15.7 kDa (predicted)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Related Tags: IL-3 Protein, Mouse, Recombinant chemical structure | IL-3 Protein, Mouse, Recombinant molecular weight