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IL-3 Protein, Rat, Recombinant (His)

(Synonyms: interleukin 3) Copy Product Info

Synonyms: interleukin 3

Catalog No. TMPY-02772 Copy Product Info
IL-3 Protein, Rat, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 17.5 kDa and the accession number is P97688.
Pack SizePriceUSA StockGlobal StockQuantity
5 µg$59-In Stock
10 µg$94-In Stock
20 µg$1557-10 days7-10 days
50 µg$3067-10 days7-10 days
100 µg$5207-10 days7-10 days
200 µg$8587-10 days7-10 days
500 µg$1,7207-10 days7-10 days
1 mg$3,5307-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Batch Information

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Purity:> 95 % as determined by SDS-PAGE
Appearance:Lyophilized powder
Color:White
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Product Introduction

Bioactivity
Bioactivity
Measured in a cell proliferation assay using FDC-P1 cells. The ED50 for this effect is typically 2-8 ng/mL.
Description
IL-3 Protein, Rat, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 17.5 kDa and the accession number is P97688.
Species
Rat
Expression System
Baculovirus Insect Cells
TagC-His
Accession NumberP97688
ConstructionA DNA sequence encoding the rat IL3 (NP_113701.1) (Met 1-Cys 169) was fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Ile 27
Protein Purity
> 95 % as determined by SDS-PAGE
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, pH 7.0, 10% gly. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionA Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonymsinterleukin 3
Research Background
IL3 (interleukin 3), also known as IL-3, is a potent growth-promoting cytokine that belongs to the IL-3 family. IL3/IL-3 also belongs to the group of interleukins. Interleukins are produced by a wide variety of body cells. The function of the immune system depends in a large part on interleukins, and rare deficiencies of a number of them have been described, all featuring autoimmune diseases or immune deficiency. The majority of interleukins are synthesized by helper CD4+ T lymphocytes, as well as through monocytes, macrophages, and endothelial cells. They promote the development and differentiation of T, B, and hematopoietic cells. IL3/IL-3 is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation, and apoptosis. IL3/IL-3 has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.
Chemical Properties
Molecular Weight17.5 kDa (predicted); 24 kDa (reducing conditions)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Related Tags: IL-3 Protein, Rat, Recombinant (His) chemical structure | IL-3 Protein, Rat, Recombinant (His) molecular weight