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G-CSF Protein, Human, Recombinant

Catalog No. TMPY-04829
Synonyms: G-CSF, colony stimulating factor 3 (granulocyte), CSF3OS, C17orf33OS, GCSF, C17orf33

G-CSF Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 18.7 kDa and the accession number is P09919-2.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
G-CSF Protein, Human, Recombinant
Pack Size Availability Price/USD Quantity
20 μg In stock $ 205.00
100 μg 5 days $ 498.00
200 μg 5 days $ 876.00
500 μg 5 days $ 1,840.00
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Biological Description
Technical Params
Product Properties
References and Literature
Biological Information Measured in a cell proliferation assay using NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 40-200pg/mL.

Description G-CSF Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 18.7 kDa and the accession number is P09919-2.
Species Human
Expression System HEK293 Cells
Tag Tag Free
Accession Number P09919-2
Synonyms G-CSF, colony stimulating factor 3 (granulocyte), CSF3OS, C17orf33OS, GCSF, C17orf33
Construction The Human CSF3 (NP_757373.1) (Met1-Pro204) was expressed with an initial Met.
Protein Purity ≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Molecular Weight 18.7 kDa (predicted)
Endotoxin < 10 EU/mg of the protein.
Formulation Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Shipping

In general, Lyophilized powders are shipping with blue ice.

Research Background Granulocyte-colony stimulating factor (G-CSF) is a growth factor and an essential cytokine belonging to the CSF family of hormone-like glycoproteins. It is produced by numerous cell types including immune and endothelial cells. G-CSF binding to its receptor G-CSF-R which belongs to the cytokine receptor type I family depends on the interaction of alpha-helical motifs of the former and two fibronectin type III as well as an immunoglobulin-like domain of the latter. Recent animal studies have also revealed that G-CSF activates multiple signaling pathways, such as Akt and also the Janus family kinase-2 and signal transducer and activation of transcription-3 (Jak2-STAT3) pathway, thereby promoting survival, proliferation, differentiation and mobilisation of haematopoietic stem and progenitor cells. G-CSF is a cytokine that have been demonstrated to improve cardiac function and perfusion in myocardial infarction. And it was initially evaluated as a stem cell mobilizer and erythropoietin as a cytoprotective agent. G-CSF prevents left ventricular remodeling after myocardial infarction by decreasing cardiomyocyte death and by increasing the number of blood vessels, suggesting the importance of direct actions of G-CSF on the myocardium rather than through mobilization and differentiation of stem cells. Accordingly, recombinant human (rh)G-CSF has been extensively used in clinical haematology and oncology to enable bone marrow transplantation or to treat chemotherapy-associated neutropenia. In preclinical study, G-CSF improved cardiac function and perfusion by angiomyogenesis and protection of cardiomyocytes in myocardial infarction.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

References and Literature

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Keywords

G-CSF Protein, Human, Recombinant G-CSF colony stimulating factor 3 (granulocyte) granulocyte CSF3OS C17orf33OS GCSF colony stimulating factor 3 C17orf33 recombinant recombinant-proteins proteins protein

 

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