G-CSF Protein, Human, Recombinant (Isoform 2)

Name: G-CSF Protein, Human, Recombinant (Isoform 2)
Brand: TargetMol
SKU: TMPY-04829
Price: 77 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: G-CSF, GCSF, CSF3OS, colony stimulating factor 3 (granulocyte), C17orf33OS, C17orf33) Copy Product Info

Synonyms: G-CSF, GCSF, CSF3OS, colony stimulating factor 3 (granulocyte), C17orf33OS, C17orf33

Catalog No. TMPY-04829 Copy Product Info

G-CSF Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 18.7 kDa and the accession number is P09919-2.

G-CSF Protein, Human, Recombinant (Isoform 2)

Pack Size	Price	USA Stock	Global Stock
5 µg	$77	-	In Stock
10 µg	$125	-	In Stock
20 µg	$205	In Stock	In Stock
50 µg	$339	7-10 days	7-10 days
100 µg	$498	-	In Stock
200 µg	$876	7-10 days	7-10 days
500 µg	$1,830	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:SDS-PAGE: 93.2%; SEC-HPLC: 98.8%

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Measured in a cell proliferation assay using NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 40-200pg/mL.
Description	G-CSF Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 18.7 kDa and the accession number is P09919-2.
Species	Human
Expression System	HEK293 Cells
Tag	Tag Free
Accession Number	P09919-2
Construction	A DNA sequence encoding the human CSF3 (NP_757373.1) (Met1-Pro204) was expressed with an initial Met. Predicted N terminal: Thr 31
Protein Purity	≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC. ,
Endotoxin	< 10 EU/mg of the protein.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	G-CSF, GCSF, CSF3OS, colony stimulating factor 3 (granulocyte), C17orf33OS, C17orf33
Research Background	Granulocyte-colony stimulating factor (G-CSF) is a growth factor and an essential cytokine belonging to the CSF family of hormone-like glycoproteins. It is produced by numerous cell types including immune and endothelial cells. G-CSF binding to its receptor G-CSF-R which belongs to the cytokine receptor type I family depends on the interaction of alpha-helical motifs of the former and two fibronectin type III as well as an immunoglobulin-like domain of the latter. Recent animal studies have also revealed that G-CSF activates multiple signaling pathways, such as Akt and also the Janus family kinase-2 and signal transducer and activation of transcription-3 (Jak2-STAT3) pathway, thereby promoting survival, proliferation, differentiation and mobilisation of haematopoietic stem and progenitor cells. G-CSF is a cytokine that have been demonstrated to improve cardiac function and perfusion in myocardial infarction. And it was initially evaluated as a stem cell mobilizer and erythropoietin as a cytoprotective agent. G-CSF prevents left ventricular remodeling after myocardial infarction by decreasing cardiomyocyte death and by increasing the number of blood vessels, suggesting the importance of direct actions of G-CSF on the myocardium rather than through mobilization and differentiation of stem cells. Accordingly, recombinant human (rh)G-CSF has been extensively used in clinical haematology and oncology to enable bone marrow transplantation or to treat chemotherapy-associated neutropenia. In preclinical study, G-CSF improved cardiac function and perfusion by angiomyogenesis and protection of cardiomyocytes in myocardial infarction.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

Chemical Properties

Molecular Weight

18.7 kDa (predicted); 19 kDa (reducing conditions)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.