DKK1 Protein, Human, Recombinant (His)

Name: DKK1 Protein, Human, Recombinant (His)
Brand: TargetMol
SKU: TMPY-00775
Rating: 4.5 (1 reviews)

(Synonyms: SK, DKK-1, dickkopf WNT signaling pathway inhibitor 1) Copy Product Info

Synonyms: SK, DKK-1, dickkopf WNT signaling pathway inhibitor 1

Catalog No. TMPY-00775 Copy Product Info

Purity: 96.7%

DKK1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 27.22 kDa and the accession number is I1W660.

Pack Size	Price	USA Stock	Global Stock
5 µg	$90	7-10 days	7-10 days
10 µg	$145	7-10 days	7-10 days
20 µg	$240	In Stock	In Stock
50 µg	$438	7-10 days	7-10 days
100 µg	$700	7-10 days	7-10 days
200 µg	$1,200	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

Add to Cart

Add to Quotation

For research use only—not for human use. No sales to individuals. Use as intended only.

Questions

Batch Information

Select Batch

Purity:96.7%

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T1/2 cells. The ED50 for this effect is approximately 0.1-0.4 μg/ml in the presence of 10 ng/mL of mouse Wnt3a.
Description	DKK1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 27.22 kDa and the accession number is I1W660.
Species	Human
Expression System	HEK293 Cells
Tag	C-His
Accession Number	I1W660
Construction	A DNA sequence encoding the human DKK1 precursor (NP_036374.1) (Met 2-His 266) was expressed with a C-terminal polyhistidine tag. Predicted N terminal: Thr 32
Protein Purity	> 90 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	SK, DKK-1, dickkopf WNT signaling pathway inhibitor 1
Research Background	Dickkopf (DKK) family proteins, consisting of DKK-1, DKK-2, DKK-3 and DKK-4, function as secreted Wnt antagonists by inhibiting Wnt coreceptors LRP5/6. DKK-1, DKK-2, and DKK-4 also bind cell surface Kremen-1 or Kremen-2 and promote the internalization of LRP5/6. Dickkopf related protein 1 (DKK-1) was initially identified as an inducer of head formation in Xenopus embryos. DKK-1 protein modulates Wnt signaling pathway during embryonic development. Increased levels of DKK-1 are found in the majority of lung cancers, esophageal squamous cell carcinomas, and hormone-resistant breast cancers, while DKK-1 expression is decreased in malignant melanoma and colorectal cancers.

Chemical Properties

Molecular Weight

27.22 kDa (predicted); 42.9 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.