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DKK1 Protein, Human, Recombinant (His)
(Synonyms: SK, DKK-1, dickkopf WNT signaling pathway inhibitor 1) Copy Product InfoSynonyms: SK, DKK-1, dickkopf WNT signaling pathway inhibitor 1
Catalog No. TMPY-00775 Copy Product Info
Purity: 96.7%
DKK1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 27.22 kDa and the accession number is I1W660.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $90 | 7-10 days | 7-10 days | |
| 10 μg | $145 | 7-10 days | 7-10 days | |
| 20 μg | $240 | In Stock | In Stock | |
| 50 μg | $438 | 7-10 days | 7-10 days | |
| 100 μg | $700 | 7-10 days | 7-10 days | |
| 200 μg | $1,200 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
Batch Information
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Purity:96.7%
Appearance:Lyophilized powder
Color:White
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T1/2 cells. The ED50 for this effect is approximately 0.1-0.4 μg/ml in the presence of 10 ng/mL of mouse Wnt3a.  |
| Description | DKK1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 27.22 kDa and the accession number is I1W660. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | C-His |
| Accession Number | I1W660 |
| Construction | A DNA sequence encoding the human DKK1 precursor (NP_036374.1) (Met 2-His 266) was expressed with a C-terminal polyhistidine tag. Predicted N terminal: Thr 32 |
| Protein Purity | > 90 % as determined by SDS-PAGE  |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | SK, DKK-1, dickkopf WNT signaling pathway inhibitor 1 |
| Research Background |
| Molecular Weight | 27.22 kDa (predicted); 42.9 kDa (reducing condition, due to glycosylation) |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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Dose Conversion
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Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: DKK1 Protein, Human, Recombinant (His) chemical structure | DKK1 Protein, Human, Recombinant (His) molecular weight
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