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DKK1 N terminal Domain Protein, Human, Recombinant (hFc & Avi), Biotinylated

Catalog No. TMPK-00158
Synonyms: hDkk1, Dkk1, Dickkopf-1, Dkk-1, Dickkopf-related 1, SK, hDkk-1

Dickkopf-1 (Dkk1), the founding and best-studied member of the Dkk family, functions as an antagonist of canonical Wnt/β-catenin. Dkk1 is considered to play a broad role in a variety of biological processes.

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DKK1 N terminal Domain Protein, Human, Recombinant (hFc & Avi), Biotinylated
Pack Size Availability Price/USD Quantity
100 μg 5 days $ 814.00
500 μg 5 days $ 3,250.00
1 mg 5 days $ 5,440.00
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Biological Description
Technical Params
Product Properties
References and Literature
Description Dickkopf-1 (Dkk1), the founding and best-studied member of the Dkk family, functions as an antagonist of canonical Wnt/β-catenin. Dkk1 is considered to play a broad role in a variety of biological processes.
Species Human
Expression System HEK293
Tag C-hFc-Avi
Accession Number O94907
Synonyms hDkk1, Dkk1, Dickkopf-1, Dkk-1, Dickkopf-related 1, SK, hDkk-1
Construction Recombinant Biotinylated Human DKK1 N terminal Domain Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus. It contains Thr32-Asp142.[Accession |O94907]
Protein Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight The protein has a predicted MW of 40.25 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
Endotoxin Less than 1EU per μg by the LAL method.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability & Storage

-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Research Background Dickkopf-1 (Dkk1), the founding and best-studied member of the Dkk family, functions as an antagonist of canonical Wnt/β-catenin. Dkk1 is considered to play a broad role in a variety of biological processes.

References and Literature

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Keywords

DKK1 N terminal Domain Protein, Human, Recombinant (hFc & Avi), Biotinylated hDkk1 Dkk1 Dickkopf-1 Dkk-1 Dickkopf-related 1 SK hDkk-1 recombinant recombinant-proteins proteins protein

 

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