CHUK/IKK alpha Protein, Human, Recombinant (GST)

Name: CHUK/IKK alpha Protein, Human, Recombinant (GST)
Brand: TargetMol
SKU: TMPC-00179
Rating: 4.5 (1 reviews)

(Synonyms: Inhibitor of nuclear factor kappa-B kinase subunit alpha, I-kappa-B kinase 1, CHUK) Copy Product Info

Synonyms: Inhibitor of nuclear factor kappa-B kinase subunit alpha, I-kappa-B kinase 1, CHUK

Catalog No. TMPC-00179 Copy Product Info

CHUK/IKK alpha Protein, Human, Recombinant (GST) is expressed in Baculovirus-Insect Cells. The accession number is Q562E6.

Pack Size	Price	USA Stock	Global Stock	Quantity
10 µg	$1,390	35 days	35 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	The specific activity of IKKα was determined to be 1.2-5.2 nmol/min/mg by ADP-Glo kinase assay.
Description	CHUK/IKK alpha Protein, Human, Recombinant (GST) is expressed in Baculovirus-Insect Cells. The accession number is Q562E6.
Species	Human
Expression System	Baculovirus-Insect Cells
Tag	N-GST
Accession Number	Q562E6
Construction	Recombinant full length human IKK alpha was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Protein Purity	>80% as determined by SDS-PAGE.
Formulation	Supplied as sterile 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Synonyms	Inhibitor of nuclear factor kappa-B kinase subunit alpha, I-kappa-B kinase 1, CHUK
Research Background	CHUK (conserved helix-loop-helix ubiquitous kinase) is ubiquitously expressed in diverse types of established cell lines, whereas it is differentially expressed in normal murine tissues. The structural features of the CHUK polypeptide suggest that its putative kinase activity may be targetted to H-L-H and/or leucine zipper transcription factors. Alternatively, the dual amphipathic a-helices may serve to control its intrinsic kinase activity by interactions with other cellular factors. CHUK may provide new insights into the regulated transmission of cytoplasmic signals to specific nuclear factors manifesting rapid alterations in patterns of cellular gene expression. The non-canonical NF-κB activating kinase IKKα, encoded by CHUK, modulates pro- or anti-inflammatory responses, cellular survival, and cellular differentiation. CHUK has an essential role in the development of the skin epidermis and its derivatives, along with various other morphogenetic events.

Chemical Properties

Molecular Weight

~114 kDa

Storage & Solubility Information

Shipping	Enzymes are highly recommended to be shipped at frozen temperature with dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.