The EC 50 value of humanMIP-3 alpha/CCL20 on Ca 2+ mobilization assay in CHO-K1/Ga15/hCCR6 cells (human Ga15 and human CCR6 stably expressed in CHO-K1 cells) is less than 200.0 ng/ml.

CCL20 Protein, Human, Recombinant (CHO) is expressed in CHO Cells. The accession number is P78556.

Human

CHO Cells

ST38,SCYA20,MIP-3-alpha,MIP-3a,MIP3A,LARC,Exodus,CKb4,chemokine (C-C motif) ligand 20,CCL-20,C-C motif chemokine ligand 20

Ala27-Met96

> 98% as determined by SDS-PAGE

Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Macrophage Inflammatory Protein-3 (MIP-3α), also known as chemokine (C-C motif) ligand 20 (CCL20) or liver activation regulated chemokine (LARC), is a small cytokine belonging to the CC chemokine family. MIP-3α is expressed in the liver, lymph nodes, appendix, PBL and lung and can signal through the CCR6 receptor. It is strongly chemotactic for lymphocytes and weakly attracts neutrophils. MIP-3α is implicated in the formation and function of mucosal lymphoid tissues via chemoattraction of lymphocytes and dendritic cells toward the epithelial cells surrounding these tissues. Additionally, it promotes the adhesion of memory CD4 + T cells and inhibits colony formation of bone marrow myeloid immature progenitors.