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Phosphatase Inhibitor Cocktail III (2 Tubes, 100x)

Catalog No. C0004

It is easy for the proteins to be degraded or dephosphorylated during in vitro extraction procedure, which could cause an inaccurate result of protein expression detection. Therefore, adding protease inhibitors or phosphatase inhibitors into the extracts would be an effective method to prevent degradation and dephosphorylation of protein.

The phosphatase inhibitor mixture can effectively inhibit the dephosphorylation of common phosphatases on proteins and maintain the original phosphorylation state of proteins.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
Phosphatase Inhibitor Cocktail III (2 Tubes, 100x)
Pack Size Availability Price/USD Quantity
200 μL In stock Inquiry Inquiry
1 mL I + 1 mL II In stock $ 43.00
(1 mL I + 1 mL II) * 10 In stock $ 321.00
(1 mL I + 1 mL II) * 50 In stock $ 1,263.00
(1 mL I + 1 mL II) * 100 Inquiry $ 1,853.00 Inquiry
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Composition

Tube No. Ingredient CAS MW Concentration (100x) Target Type
I (ddH2O) 1mL Sodium Fluoride 7681-49-4 41.99 100 mM Acid Phosphatase Reversible
Sodium Orthovanadate 13721-39-6 183.91 100 mM Alkaline phosphatase, PTPs, ATPases Reversible
Sodium Molybdate 7631-95-0 205.92 115 mM Acid and phosphoprotein Phosphatase Irreversible
Sodium Tartrate 868-18-8 194.05 400mM Acid Phosphatase Reversible
Imidazole 288-32-4 68.08 200 mM Alkaline phosphatase Reversible
II (DMSO) 1mL (-)-p-Bromotetramisole oxalate 62284-79-1 373.22 2.5 mM Alkaline phosphatases Irreversible
Cantharidin 56-25-7 196.2 500 μM Ser/Thr phosphatases Reversible
Microcystin LR 101043-37-2 995.17 500 nM Acid and PP1 and PP2A Reversible

Features

  • Accurate Ingredients —— Clearance of components and concentrations to avoid experimental uncertainty
  • Comprehensive Protection—— Effectively inhibit various proteases to protect proteins from being degraded
  • Cost-Effective —— Lower price with higher quality

Handling Instruction

  • Compatible with Western Blot analysis, IP, Co-IP, pull-down, IF, IHC, kinase assay and etc.
  • Add concentrated cocktail at 1:100 (v/v) dilution to samples solution (such as cell lysates or tissue extracts) before assaying.
  • Briefly vortex cocktails to help facilitate the dissolution.
  • Note to add tube I first, mix well, then add tube II, mix again.

Notice

  • Following initial thaw, aliquot and freeze (-20°C) to avoid thawing and refreezing repeatedly.
  • If Cocktail I and Cocktail II are used together, do not mix tube I with tube II beforehand, as there may be precipitation. To avoid this, please add them step by step during experiment.

Citations

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11. Su J, Thakur A, Pan G, et al.Morus alba derived Kuwanon: A combined with 5‐fluorouracil reduce tumor progression via synergistic activation of GADD153 in gastric cancer.MedComm–Oncology.2023, 2(1): e24. 12. Hu H, Xu L, Luo S J, et al. Retinal dehydrogenase 5 (RHD5) attenuates metastasis via regulating HIPPO/YAP signaling pathway in Hepatocellular Carcinoma. International Journal of Medical Sciences. 2020, 17(13): 1897 13. Hu H, Luo S J, Cao Z, et al. Depressive Disorder promotes Hepatocellular Carcinoma metastasis via upregulation of ABCG2 gene expression and maintenance of self-renewal. Journal of Cancer. 2020, 11(18): 5309-5317 14. Zhong L, Ren X, Ai Y, et al. SS-31 Improves Cognitive Function in Sepsis-Associated Encephalopathy by Inhibiting the Drp1-NLRP3 Inflammasome Activation. NeuroMolecular Medicine. 2022: 1-12. 15. Tang H, Guo J, Sang B, et al. PDGFRβ Modulates Aerobic Glycolysis in Osteosarcoma HOS Cells via the PI3K/AKT/mTOR/c-Myc Pathway. Biochemistry and Cell Biology. 2021 (ja). 16. Liu H, Mi Q, Ding X, et al. Discovery and characterization of novel potent BCR-ABL degraders by conjugating allosteric inhibitor. European Journal of Medicinal Chemistry. 2022: 114810 17. Xue D D, Wei C L, Zhou Y H, et al. TRIOL Inhibits Rapid Intracellular Acidification and Cerebral Ischemic Injury: The Role of Glutamate in Neuronal Metabolic Reprogramming. ACS Chemical Neuroscience. 2022 18. Du T, Zhang Z, Zhou J, et al. A Novel PARP Inhibitor YHP-836 For the Treatment of BRCA-Deficiency Cancers. Frontiers in pharmacology. 2022, 13. 19. Shi Y, Cui X, Jiang T, et al. The therapeutic effect of KSP inhibitors in preclinical models of cholangiocarcinoma. Cell Death & Disease. 2022, 13(9): 1-13. 20. Zhang F, Hu G, Chen X, et al. Excessive branched-chain amino acid accumulation restricts mesenchymal stem cell-based therapy efficacy in myocardial infarction. Signal Transduction and Targeted Therapy. 2022, 7(1): 1-18 21. Li L, Xu X, Lv K, et al. Asebogenin Suppresses Thrombus Formation via Inhibition of Syk Phosphorylation. British Journal of Pharmacology. 2022 22. Lu B, Zhu Z, Sheng L, et al. SMARCB1 Promotes Ubiquitination and Degradation of NR4A3 via Direct Interaction Driven by ROS in Vascular Endothelial Cell Injury. Oxidative medicine and cellular longevity. 2020 Oct 23;2020:2048210 23. Niu Z, Wang L, Qin X, et al. Macrophage derived miR-7219-3p-containing exosomes mediate fibroblast trans-differentiation by targeting SPRY1 in silicosis. Toxicology. 2022: 153310. 24. Wu F, Xie X, Li G, et al. AKG induces cell apoptosis by inducing reactive oxygen species‐mediated endoplasmic reticulum stress and by suppressing PI3K/AKT/mTOR‐mediated autophagy in renal cell carcinoma. Environmental Toxicology. 2022 25. Hu S, Wang Z, Jin C, et al. Human amniotic epithelial cell-derived extracellular vesicles provide an extracellular matrix-based microenvironment for corneal injury repair. Journal of Tissue Engineering. 2022, 13: 20417314221122123 26. Li H, Liu N N, Li J R, et al. Combined Use of Bicyclol and Berberine Alleviates Mouse Nonalcoholic Fatty Liver Disease. Frontiers in pharmacology. 2022, 13: 843872-843872. 27. Guo D, Yang X, Gao M, et al. Deficiency of Autism-Related Gene Dock4 Leads to Impaired Spatial Memory and Hippocampal Function in Mice at Late Middle Age. Cellular and Molecular Neurobiology. 2022: 1-18 28. Li H, Liu N N, Li J R, et al. Bicyclol ameliorates advanced liver diseases in murine models via inhibiting the IL-6/STAT3 signaling pathway. Biomedicine & Pharmacotherapy. 2022, 150: 113083 29. Xiong T C, Wei M C, Li F X, et al. The E3 ubiquitin ligase ARIH1 promotes antiviral immunity and autoimmunity by inducing mono-ISGylation and oligomerization of cGAS. Nature Communications. 2022, 13(1): 1-19. 30. Li H, Liu N N, Li J R, et al. Combined Use of Bicyclol and Berberine Alleviates Mouse Nonalcoholic Fatty Liver Disease. Frontiers in pharmacology. 2022, 13: 843872-843872. 31. Qian H, Zhou T, Fu Y, et al. Self-assembled Tetrahedral Framework Nucleic Acid Mediated Tumor-associated Macrophages Reprogramming and Restores Antitumor Immunity. Molecular Therapy-Nucleic Acids. 2022 32. Jin C, Ou Q, Chen J, et al. Chaperone-mediated autophagy plays an important role in regulating retinal progenitor cell homeostasis. Stem Cell Research & Therapy. 2022, 13(1): 1-14 33. Song X, Qiao L, Chang J, et al. Dietary supplementation with selenium nanoparticles-enriched Lactobacillus casei ATCC 393 alleviates intestinal barrier dysfunction of mice exposed to deoxynivalenol by regulating endoplasmic reticulum stress and gut microbiota. Ecotoxicology and Environmental Safety. 2022, 248: 114276.
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