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Zinpyr-1

Name: Zinpyr-1
Brand: TargetMol
SKU: TYD-00504
Price: 46 USD
Availability: InStock
Rating: 5 (10 reviews)

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Purity: 98.44%

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Zinpyr-1 is a zinc ion-responsive fluorescent indicator and cell membrane-permeable metal-binding probe. It binds to Mn²⁺ to form a complex with enhanced fluorescence signals. Moreover, Zinpyr-1 specifically binds to free zinc ions in serum, enabling quantitative determination of free zinc ion concentration. In plant systems, the fluorescence signal of Zinpyr-1 reflects the relative zinc ion level in root cells and is mainly localized in specific regions of root cells. It can be applied to the screening of plant zinc transporter mutants and research on zinc homeostasis regulation.

Cas No. 288574-78-7

Pack Size	Price	USA Stock	Global Stock	Quantity
1 mg	$46	-	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:98.44%

Appearance:Solid

Color:Pink

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COA LCMS

Product Introduction

Bioactivity

Description

In vivo

Methods: A complex was constructed using Zinpyr-1 and Mn²⁺, and its fluorescence response characteristics to Zn²⁺ were detected under different pH conditions. Cell experiments were performed to evaluate its membrane permeability and cytotoxicity, and its capability for zinc ion detection and imaging was verified in serum system and Arabidopsis thaliana seedling model.
Results:
1.Zinpyr-1 (10 μM, pH 6–8) could form a 1:2 complex with Mn²⁺. When used for Zn²⁺ detection, the fluorescence dynamic range was 25 times higher than that of the free ligand (106 at pH 7.0).It still maintained a high dynamic range at pH 6.0 and 8.0, and exhibited significantly better selectivity for Zn²⁺ than common monovalent and divalent cations in vivo.
2.The complex formed by Zinpyr-1 (10 μM) and Mn²⁺ possessed cell membrane permeability and showed no cytotoxicity to HeLa cells. It had extremely low background fluorescence and was remarkably activated after binding to Zn²⁺, which was suitable for zinc ion imaging in living cells.
3.The complex formed by Zinpyr-1 (0.1 mM) and Mn²⁺ could be used as a zinc-responsive MRI contrast agent [1].
4.Zinpyr-1 could stably produce the minimum fluorescence (Fmin) and maximum fluorescence (Fmax) for the quantification of free zinc in serum, with a maximum excitation wavelength of 507 nm and a maximum emission wavelength of 526 nm, enabling reliable quantification of serum free zinc [2].
5.Zinpyr-1 (5–20 μM, 15 min–3 h) could serve as a fluorescent probe for relative zinc levels in root cells of Arabidopsis thaliana seedlings, and the fluorescence intensity was positively correlated with intracellular zinc concentration [3].

Chemical Properties

Molecular Weight	823.72
Formula	C₄₆H₃₆Cl₂N₆O₅
Cas No.	288574-78-7
Smiles	O=C1OC2(C=3C=CC=CC13)C4=CC(Cl)=C(O)C(=C4OC=5C2=CC(Cl)=C(O)C5CN(CC6=NC=CC=C6)CC7=NC=CC=C7)CN(CC8=NC=CC=C8)CC9=NC=CC=C9

Storage & Solubility Information

Storage

Keep away from direct sunlight,Store under nitrogen Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 16.00 mg/mL (19.42 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.2140 mL	6.0700 mL	12.1400 mL	60.7002 mL
5 mM	0.2428 mL	1.2140 mL	2.4280 mL	12.1400 mL
10 mM	0.1214 mL	0.6070 mL	1.2140 mL	6.0700 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Related Tags: Zinpyr-1 chemical structure | Zinpyr-1 in vivo | Zinpyr-1 formula | Zinpyr-1 molecular weight