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V5 Epitope Tag Peptide

Name: V5 Epitope Tag Peptide
Brand: TargetMol
SKU: TP2189
Rating: 4.5 (1 reviews)

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Catalog No. TP2189

The recognized V5 epitope represents 95GKPIPNPLLGLDST108 of RNA polymerase α subunit of simian parain uenza virus type 5. This short peptide sequence was chosen because high-af nity antibodies can be reliably produced in many different species1.

V5 Epitope Tag Peptide

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Catalog No. TP2189

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
5 mg	$115	Inquiry	Inquiry

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Product Introduction

Bioactivity

Description	The recognized V5 epitope represents 95GKPIPNPLLGLDST108 of RNA polymerase α subunit of simian parain uenza virus type 5. This short peptide sequence was chosen because high-af nity antibodies can be reliably produced in many different species1.
In vitro	A 14-amino-acid V5 epitope derived from simian parain?uenza virus type 5 was inserted into the C terminus of the capsid protein (before the stop codon ofORF2) to construct a recombinant marker virus. We demonstrated that the V5 epitope was displayed on the surface of the capsid protein. Furthermore, the recombinant marker virus behaved similarly to the parental virus in vitro and in mice and could be differentiated from the parental virus via polymerase chain reaction (PCR) and serological methods [2][3].
In vivo	A V5/His-epitope tag was added to the amino terminus of each coding sequence to distinguish the plasmid-encoded protein from endogenous LRRK2. Plasmids were transiently transfected into COS7 cells, and cell lysates were analyzed by SDS-PAGE and Western blotting with antibodies speci?c for the V5 epitope tag or LRRK24.

Chemical Properties

Molecular Weight	1421.64
Formula	C₆₄H₁₀₈N₁₆O₂₀
Smiles	CCC(C)C(C(=O)N1CCCC1C(=O)NC(CC(=O)N)C(=O)N2CCCC2C(=O)NC(CC(C)C)C(=O)NC(CC(C)C)C(=O)NCC(=O)NC(CC(C)C)C(=O)NC(CC(=O)O)C(=O)NC(CO)C(=O)NC(C(C)O)C(=O)O)NC(=O)C3CCCN3C(=O)C(CCCCN)NC(=O)CN
Relative Density.	no data available
Sequence	H-Gly-DL-Lys-DL-Pro-DL-xiIle-DL-Pro-DL-Asn-DL-Pro-DL-Leu-DL-Leu-Gly-DL-Leu-DL-Asp-DL-Ser-DL-xiThr-OH
Sequence Short	GKPXPNPLLGLDSX

Storage & Solubility Information

Storage	keep away from moisture \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Solubility Information	H2O: ≥55.4 mg/mL, Sonication is recommended. DMSO: ≥71.082 mg/mL, Sonication is recommended. Ethanol: ≥107.2 mg/mL, Sonication is recommended.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

V-5 Epitope Tag PeptideV5 Epitope Tag Peptide