Shopping Cart

Remove All

Your shopping cart is currently empty

Proceed to checkout Continue shopping

TTFB

Name: TTFB
Brand: TargetMol
SKU: T218004
Rating: 4.5 (1 reviews)

(Synonyms: N-(4-tert-butyl-1,3-thiazol-2-yl)-3-fluorobenzamide) Copy Product Info

🥰Excellent

Synonyms: N-(4-tert-butyl-1,3-thiazol-2-yl)-3-fluorobenzamide

Catalog No. T218004 Copy Product Info

🥰Excellent

TTFB is a selective, non-competitive zinc-activated channel (ZAC) antagonist. It inhibits Zn²⁺ and H⁺-induced ZAC currents with IC₅₀ values of 3 μM and 8.5 μM, respectively, and has an IC₅₀ of 4.7 μM against spontaneous activity. TTFB does not show significant agonistic, antagonistic, or modulatory activity on classic Cys-loop receptor representatives such as m5-HT3AR, hα3β4 nAChR, hα1β2γ2S GABAAR, and hα1 GlyR. It is used to study the physiological and pathological functions of ZAC.

TTFB

Cas No. 352560-76-0

Pack Size	Price	USA Stock	Global Stock	Quantity
10 mg	Inquiry	10-14 weeks	10-14 weeks
50 mg	Inquiry	10-14 weeks	10-14 weeks

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

Add to Quotation

For research use only—not for human use. No sales to individuals. Use as intended only.

Questions

Resource Download

Product Introduction

Bioactivity

Description	TTFB is a selective, non-competitive zinc-activated channel (ZAC) antagonist. It inhibits Zn²⁺ and H⁺-induced ZAC currents with IC₅₀ values of 3 μM and 8.5 μM, respectively, and has an IC₅₀ of 4.7 μM against spontaneous activity. TTFB does not show significant agonistic, antagonistic, or modulatory activity on classic Cys-loop receptor representatives such as m5-HT3AR, hα3β4 nAChR, hα1β2γ2S GABAAR, and hα1 GlyR. It is used to study the physiological and pathological functions of ZAC.
In vitro	TTFB (0.1-100 μM; 30 seconds) effectively inhibits Zn²⁺-induced, H⁺-induced, and spontaneous ZAC activity in Xenopus oocytes, with IC₅₀ values of 3.0 μM, 8.5 μM, and 4.7 μM, respectively, and can fully mediate the inhibition of the Zn²⁺-induced response. TTFB (10 μM) mediates voltage-independent ZAC inhibition, showing similar inhibitory effects on H⁺-induced currents at -60 mV and +60 mV.
Synonyms	N-(4-tert-butyl-1,3-thiazol-2-yl)-3-fluorobenzamide

Chemical Properties

Molecular Weight	278.35
Formula	C₁₄H₁₅FN₂OS
Cas No.	352560-76-0
Smiles	O=C(NC1=NC(=CS1)C(C)(C)C)C=2C=CC=C(F)C2

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Related Tags: TTFB in vitro | TTFB formula | TTFB molecular weight