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SYBR Green II (Ionic form)

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Catalog No. T208437Cas No. 178919-00-1

SYBR Green II (Ionic form) is a fluorescent dye for nucleic acids that primarily binds to single-stranded nucleotides. It is sensitive to oligonucleotides and larger nucleic acid polymers in various cells and gels. SYBR Green II is applicable in studies of cellular structure, membrane integrity or functionality, and cell cycle distribution. Wavelength: 484/515 nm.

SYBR Green II (Ionic form)

SYBR Green II (Ionic form)

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Catalog No. T208437Cas No. 178919-00-1
SYBR Green II (Ionic form) is a fluorescent dye for nucleic acids that primarily binds to single-stranded nucleotides. It is sensitive to oligonucleotides and larger nucleic acid polymers in various cells and gels. SYBR Green II is applicable in studies of cellular structure, membrane integrity or functionality, and cell cycle distribution. Wavelength: 484/515 nm.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
10 mgInquiry10-14 weeks10-14 weeks
50 mgInquiry10-14 weeks10-14 weeks
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
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Product Introduction

Bioactivity
Description
SYBR Green II (Ionic form) is a fluorescent dye for nucleic acids that primarily binds to single-stranded nucleotides. It is sensitive to oligonucleotides and larger nucleic acid polymers in various cells and gels. SYBR Green II is applicable in studies of cellular structure, membrane integrity or functionality, and cell cycle distribution. Wavelength: 484/515 nm.
In vitro
SYBR Green II binds to RNA or single-stranded DNA, emitting fluorescence. It can be used for staining RNA/DNA either before or after electrophoresis.
Chemical Properties
Molecular Weight454.61
FormulaC28H28N3OS
Cas No.178919-00-1
SmilesO1C=2C=CC=CC2N(C1=CC=3C=C(SCCN(C)C)[N+](C=4C=CC=CC4)=C5C=CC=CC35)C
Storage & Solubility Information
Storagekeep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
TargetMol | Animal experiments For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.
Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSOTargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSOTargetMol | reagent stock solution to 400 μL PEG300TargetMol | reagent and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH2OTargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.
1 Enter information below:
mg/kg
g
μL
2 Enter the in vivo formulation:
% DMSO
%
% Tween 80
% Saline/PBS/ddH2O

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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