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Sodium Citrate Buffer, 0.5M, pH 6.5
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Catalog No. TSH-00286
Sodium Citrate Buffer, 0.5M, pH 6.5, is a commonly used buffer solution composed mainly of citric acid and disodium hydrogen phosphate. It is employed in heat-induced epitope retrieval (HIER) to restore antigenicity in formalin-fixed, paraffin-embedded tissues where some epitope antigenicity has been lost. This buffer is the preferred solution for most antibodies.
Sodium Citrate Buffer, 0.5M, pH 6.5
Sodium Citrate Buffer, 0.5M, pH 6.5
😃Good
Catalog No. TSH-00286
Sodium Citrate Buffer, 0.5M, pH 6.5, is a commonly used buffer solution composed mainly of citric acid and disodium hydrogen phosphate. It is employed in heat-induced epitope retrieval (HIER) to restore antigenicity in formalin-fixed, paraffin-embedded tissues where some epitope antigenicity has been lost. This buffer is the preferred solution for most antibodies.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 10 mg | Inquiry | 7-10 days | |
| 50 mg | Inquiry | 7-10 days |
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Chemical Properties
| Description | Sodium Citrate Buffer, 0.5M, pH 6.5, is a commonly used buffer solution composed mainly of citric acid and disodium hydrogen phosphate. It is employed in heat-induced epitope retrieval (HIER) to restore antigenicity in formalin-fixed, paraffin-embedded tissues where some epitope antigenicity has been lost. This buffer is the preferred solution for most antibodies. |
Storage & Solubility Information
| Storage | keep away from moisture | store at 4°C |
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In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . A total of 10 animals were administered, and the formula you used is 5% 
DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH2O. So your working solution concentration is 2 mg/mL。Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.Preparation method for in vivo formula: Take 50 μL DMSO main solution, add 300 μLPEG300 mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2O mix well and clarify
main solution, add 300 μLPEG300 mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2O mix well and clarifyFor Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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