R-Spondin 1/RSPO1 Protein, Mouse, Recombinant (His)

Catalog No. TMPY-03197

R-Spondin 1/RSPO1 Protein, Mouse, Recombinant (His) is expressed in CHO mammalian cells with His tag. The predicted molecular weight is 28.5 kDa and the accession number is B1ASC1.

R-Spondin 1/RSPO1 Protein, Mouse, Recombinant (His)

Catalog No. TMPY-03197

R-Spondin 1/RSPO1 Protein, Mouse, Recombinant (His) is expressed in CHO mammalian cells with His tag. The predicted molecular weight is 28.5 kDa and the accession number is B1ASC1.

Pack Size	Price	Availability
5 μg	$71	In Stock
10 μg	$113	In Stock
20 μg	$187	In Stock
50 μg	$368	7-10 days
100 μg	$618	In Stock
200 μg	$1,080	7-10 days
500 μg	$2,280	7-10 days
1 mg	$4,020	In Stock

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Product Information

Biological Activity	Measured by its ability to induce activation of β-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 0.1-0.9 μg/ml in the presence of 5 ng/mL recombinant mouse Wnt3a.
Description	R-Spondin 1/RSPO1 Protein, Mouse, Recombinant (His) is expressed in CHO mammalian cells with His tag. The predicted molecular weight is 28.5 kDa and the accession number is B1ASC1.
Species	Mouse
Expression System	CHO Cells
Tag	C-His
Accession Number	Q9Z132
Synonyms	R-spondin 1,R-spondin,Rspondin
Construction	A DNA sequence encoding the full length of mouse RSPO1 (NP_619624.2) (Met 1-Gln 265) was fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Ser 21
Protein Purity	> 95 % as determined by SDS-PAGE
Molecular Weight	28.5 kDa (predicted); 44 kDa (reducing condition, due to glycosylation)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	RSPO1 gene is a member of the R-spondin family. It encodes RSPO1 which is known as a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, RSPO1 induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. This protein is an activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. RSPO1 acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. It also acts as a ligand for frizzled FZD8 and LRP6.