1. Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 1-10 ng/ml
2. Mouse intestinal organoids were cultured with EGF, Noggin and R-spondin 1. After five days of culture, the organoids showed good morphology and germination rate.
3. Mouse Gastric organoids were cultured with EGF, Wnt3a, Noggin, R-spondin 1 and FGF-10. The organoids showed good morphology.
4. Mouse bile duct organoids were cultured with EGF, R-spondin 1, FGF-10 and HGF. The organoids showed good morphology.
5. Human intestinal organoids (ipsc source) were cultured with Activin A, BMP-4, EGF, Wnt3a, Noggin, R-spondin 1 and FGF-4. The organoids showed good morphology.

R-Spondin 1/RSPO1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 28.2 kDa and the accession number is Q2MKA7-1.

Human

HEK293 Cells

R-spondin 1,RSPO,CRISTIN3

Ser21-Ala263

> 95 % as determined by SDS-PAGE

Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

RSPO1 gene is a member of the R-spondin family. It encodes RSPO1 which is known as a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, RSPO1 induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. This protein is an activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. RSPO1 acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. It also acts as a ligand for frizzled FZD8 and LRP6.