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Rose bengal lactone

🥰Excellent
Catalog No. T87342Cas No. 4159-77-7
Alias Rose Bengal, C.I. Solvent Red 141

Rose bengal lactone (C.I. Solvent Red 141) is a rose bengal lactone stain commonly used to stain the nuclei of plant and animal cells.

Rose bengal lactone

Rose bengal lactone

🥰Excellent
Purity: 97.25%
Catalog No. T87342Alias Rose Bengal, C.I. Solvent Red 141Cas No. 4159-77-7
Rose bengal lactone (C.I. Solvent Red 141) is a rose bengal lactone stain commonly used to stain the nuclei of plant and animal cells.
Pack SizePriceAvailabilityQuantity
1 mg$195In Stock
5 mg$483In Stock
10 mg$692In Stock
25 mg$1,080In Stock
50 mg$1,490In Stock
100 mg$1,970In Stock
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Purity:97.25%
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Product Introduction

Bioactivity
Description
Rose bengal lactone (C.I. Solvent Red 141) is a rose bengal lactone stain commonly used to stain the nuclei of plant and animal cells.
Cell Research
Instructions
I. Dye preparation:
1. Stock solution preparation: Dissolve it in an appropriate solvent (usually water, ethanol or buffer solution) to prepare a stock solution.
2. Working solution preparation: The commonly used working concentration range is 1–10 µM, and the specific concentration can be adjusted according to experimental needs.
II. Operation steps
1. Cell preparation:
1) Inoculate the cells to be stained onto a microscope slide, culture dish or other suitable substrate.
2) Incubation: Add the prepared rose bengal lactone solution to the cells. The incubation time is usually 30 minutes to 1 hour at 37°C, but it can be optimized according to the cell type and staining intensity requirements.
3) Washing: After incubation, wash the cells with an appropriate buffer or PBS (phosphate buffered saline) to remove unbound dye.
2. Imaging and analysis:
1) After the cells are stained and washed, they can be observed under a fluorescence microscope. Rose bengal lactone usually emits strong fluorescence, which facilitates the visualization of the cell nucleus.
2) The excitation wavelength of rose bengal lactone is usually around 550 nm, and the emission wavelength is in the red to orange spectrum.
3. Data interpretation:
1) Fluorescence intensity is related to the amount of dye uptake and can be used to determine the number and state of cells.
2) It can also be used to evaluate cell morphological changes or observe specific cell structures and processes, depending on the context of the experiment.
Notes:
1. Toxicity: Rose bengal lactone is a synthetic dye and should be used with caution. It is recommended to wear gloves and goggles during operation and work in a well-ventilated area.
2. Photosensitivity: Like many fluorescent dyes, rose bengal lactone is light-sensitive, so long-term exposure to strong light should be avoided during operation and storage to prevent photobleaching.
3. Concentration control: The concentration of the dye needs to be properly controlled. Excessive staining concentration may cause high background fluorescence or cytotoxicity.
AliasRose Bengal, C.I. Solvent Red 141
Chemical Properties
Molecular Weight973.67
FormulaC20H4Cl4I4O5
Cas No.4159-77-7
SmilesO=C1OC2(C3=CC(I)=C(O)C(I)=C3OC4=C(I)C(O)=C(I)C=C42)C=5C(Cl)=C(Cl)C(Cl)=C(Cl)C15
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

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Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2OTargetMol | reagent mix well and clarify
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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