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Red-HyP-1 is a red dye that can be used for in vivo hypoxia imaging. The maximum emission maximum of Red-HyP-1 is 798 nm.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
1 mg | $195 | In Stock | |
5 mg | $483 | In Stock | |
10 mg | $692 | In Stock | |
25 mg | $1,080 | In Stock | |
50 mg | $1,490 | In Stock | |
100 mg | $1,970 | In Stock |
Description | Red-HyP-1 is a red dye that can be used for in vivo hypoxia imaging. The maximum emission maximum of Red-HyP-1 is 798 nm. |
In vitro | Under hypoxic conditions, CYP450 enzymes react with the N-oxide group, undergoing two-electron reduction to yield the uncapped dye (Red-HyP-1) which results in a red-shifted emission. HyP-1 has an absorbance and emission maxima at 670 and 697 nm, respectively, while red-Hyp-1 (the turned over product) has an absorbance and emission maxima at 760 and 798 nm. [1] |
Animal Research | Instructions 1. Solution preparation Stock solution: Dissolve Red-HyP-1 in anhydrous DMSO or DMF to prepare a 1–10 mM stock solution. Working solution: Dilute to a working concentration of 1–10 µM using an experimental buffer (e.g., PBS, pH 7.4). 2. Experimental steps In vitro experiments 1) Add Red-HyP-1 to the sample solution (e.g., cell culture medium or biological buffer). 2) Incubate for 30 minutes to 1 hour at 37°C, away from light. 3) Detect fluorescence signal: Excitation wavelength: 720–750 nm Emission wavelength: 798 nm In vivo hypoxia imaging 1. Inject Red-HyP-1 into experimental animals (e.g., tail vein injection, the specific dose depends on the experimental design, usually 1–5 mg/kg). Wait 30 minutes to 1 hour after injection and detect using a fluorescence imager. 2. Signal detection equipment Use near-infrared fluorescence imaging system or fluorescence microscope to record the signal. 3. Calibration and control 1) Control experiment: Set up control groups with normal oxygen concentration (such as 20% O₂) and low oxygen concentration (such as 1% O₂). Verify whether the signal is related to hypoxia. 2) Standard curve: Use samples under known oxygen concentration gradient to establish the relationship curve between fluorescence signal and oxygen concentration. Notes 1) Photosensitivity: Red-HyP-1 is sensitive to light. Avoid strong light exposure throughout the experiment to prevent fluorescence signal attenuation. 2) Storage conditions: Red-HyP-1 should be stored at -20°C in the dark and avoid repeated freezing and thawing. 3) Dissolution stability: Ensure that the solution is fully dissolved before use; if necessary, undissolved particles can be removed by low-speed centrifugation. |
Molecular Weight | 633.95 |
Formula | C37H33BClF2N4O |
Smiles | [Cl-].CCN(CC)c1ccc(cc1)-c1cc(c2N=C3C(=CC(c4ccc(OC)cc4)=[N+]3[B](F)(F)n12)c1ccccc1)-c1ccccc1 |
Relative Density. | no data available |
Storage | keep away from direct sunlight,keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
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