PROTACBRD4 Degrader-31 is a BRD4 PROTAC degrader with DC50 values of 164 nM at 4 hours and 80 nM at 24 hours. It effectively degrades BRD4 within cells and exhibits long-lasting degradation kinetics.

BRD4:4h, 164 nM (DC50)

PROTAC BRD4 Degrader-31 (Compound 8) significantly reduces BRD4 levels in BRD4-HiBiT transgenic HEK293T cells within 4 to 24 hours, with a DC₅₀ of 164 nM at 4 hours and 80 nM at 24 hours. Within a concentration range of 0.1-10 µM over 24 hours, it effectively degrades endogenous BRD4 protein in SK-BR-3 cells via a proteasome-mediated mechanism, achieving a maximum degradation rate of 94% at 24 hours.