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Probe-Cys

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Catalog No. T209841

Probe-Cys is a water-soluble, selective near-infrared fluorescent probe for Cys (λex= 680 nm, λem=710 nm) that remains unaffected by Hcy, GSH, and HS-. This compound can react with the stimulant 1,4-dithiothreitol (DTT) and the inhibitor N-ethylmaleimide (NEM) in HepG2 cells and zebrafish for detecting endogenous Cys. Furthermore, Probe-Cys is applicable for imaging Cys in Arabidopsis thaliana. It offers a method for exploring cancer diagnostics and plant sulfur metabolism.

Probe-Cys

Probe-Cys

Copy Product Info
😃Good
Catalog No. T209841
Probe-Cys is a water-soluble, selective near-infrared fluorescent probe for Cys (λex= 680 nm, λem=710 nm) that remains unaffected by Hcy, GSH, and HS-. This compound can react with the stimulant 1,4-dithiothreitol (DTT) and the inhibitor N-ethylmaleimide (NEM) in HepG2 cells and zebrafish for detecting endogenous Cys. Furthermore, Probe-Cys is applicable for imaging Cys in Arabidopsis thaliana. It offers a method for exploring cancer diagnostics and plant sulfur metabolism.
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
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Product Introduction

Bioactivity
Description
Probe-Cys is a water-soluble, selective near-infrared fluorescent probe for Cys (λex= 680 nm, λem=710 nm) that remains unaffected by Hcy, GSH, and HS-. This compound can react with the stimulant 1,4-dithiothreitol (DTT) and the inhibitor N-ethylmaleimide (NEM) in HepG2 cells and zebrafish for detecting endogenous Cys. Furthermore, Probe-Cys is applicable for imaging Cys in Arabidopsis thaliana. It offers a method for exploring cancer diagnostics and plant sulfur metabolism.
Chemical Properties
FormulaC33H31NO7S
Storage & Solubility Information
Storagekeep away from direct sunlight | store at -20°C | Shipping with blue ice/Shipping at ambient temperature.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
TargetMol | Animal experiments For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.
Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSOTargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSOTargetMol | reagent stock solution to 400 μL PEG300TargetMol | reagent and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH2OTargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.
1 Enter information below:
mg/kg
g
μL
2 Enter the in vivo formulation:
% DMSO
%
% Tween 80
% Saline/PBS/ddH2O

Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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