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Phos-FLAG
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Catalog No. TP4394 Copy Product Info
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Phos-FLAG is a phosphorylated FLAG peptide. It can undergo Staudinger ligation with azide groups integrated into cell surface glycans and organ glycoproteins for the quantitative analysis of cell surface azides. Phos-FLAG is also capable of detecting azide-labeled glycans in isolated cell and organ lysates.
Phos-FLAG
Cas No. 403481-00-5
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 10 mg | Inquiry | Inquiry | Inquiry | |
| 50 mg | Inquiry | Inquiry | Inquiry |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Description | Phos-FLAG is a phosphorylated FLAG peptide. It can undergo Staudinger ligation with azide groups integrated into cell surface glycans and organ glycoproteins for the quantitative analysis of cell surface azides. Phos-FLAG is also capable of detecting azide-labeled glycans in isolated cell and organ lysates. |
| In vivo | Phos-Flag (250-500 μM; ex vivo incubation; 1-12 h; room temperature) is capable of mediating a specific, detectable Staudinger ligation reaction on azide groups metabolically introduced into mouse spleen cells and organ glycoproteins, resulting in dose-dependent and tissue-specific labeling signals. Additionally, Phos-Flag (16 μmol; i.p.; single dose; 24 hours after final Ac4ManNAz injection) successfully facilitates in vivo Staudinger ligation with metabolically introduced azide groups on mouse spleen cells, producing detectable cell surface Flag epitopes without observed toxicity. |
| Molecular Weight | 1359.29 |
| Formula | C62H75N10O23P |
| Cas No. | 403481-00-5 |
| Smiles | P(C1=C(C(OC)=O)C=CC(C(N[C@H](C(N[C@@H](CC2=CC=C(O)C=C2)C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@@H](CCCCN)C(O)=O)=O)CC(O)=O)=O)CC(O)=O)=O)CC(O)=O)=O)CC(O)=O)=O)CCCCN)=O)=O)CC(O)=O)=O)=C1)(C3=CC=CC=C3)C4=CC=CC=C4 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. |
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In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Stock Solution Preparation:
Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
Preparation of the In Vivo Formulation:
1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 µL Tween 80 and mix well until fully clarified.
3) Add 450 µL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
Dose Conversion
You can also refer to dose conversion for different animals. More Dose Conversion
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: Phos-FLAG in vivo | Phos-FLAG formula | Phos-FLAG molecular weight
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