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PFB-FDG

Name: PFB-FDG
Brand: TargetMol
SKU: T208388
Rating: 4.5 (1 reviews)

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Catalog No. T208388Cas No. 209540-64-7

PFB-FDG is a non-fluorescent galactosidase substrate that can be hydrolyzed into the green fluorescent PFB-F (Ex=485 nm, Em=535 nm). It is used for measuring β-galactosidase activity.

PFB-FDG

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Catalog No. T208388Cas No. 209540-64-7

PFB-FDG is a non-fluorescent galactosidase substrate that can be hydrolyzed into the green fluorescent PFB-F (Ex=485 nm, Em=535 nm). It is used for measuring β-galactosidase activity.

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
10 mg	Inquiry	Inquiry	Inquiry
50 mg	Inquiry	Inquiry	Inquiry

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Product Introduction

Bioactivity

Description	PFB-FDG is a non-fluorescent galactosidase substrate that can be hydrolyzed into the green fluorescent PFB-F (Ex=485 nm, Em=535 nm). It is used for measuring β-galactosidase activity.
In vitro	Measurement of β-galactosidase activity involves incubating 2 × 10^5 cells with 50 μg/mL of PFB-FDG in serum-free DMEM containing 25 mM Hepes (pH 7.4) at 37℃ for 2 hours. (Mononuclear cells, for example, may require different concentrations.) Wash the cells once with ice-cold PBS and analyze using flow cytometry (Ex=485 nm, Em=535 nm).

Chemical Properties

Molecular Weight	865.66
Formula	C₃₉H₃₂F₅NO₁₆
Cas No.	209540-64-7
Smiles	O=C1OC2(C=3C1=CC(NC(=O)C4=C(F)C(F)=C(F)C(F)=C4F)=CC3)C=5C(OC=6C2=CC=C(O[C@H]7[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O7)C6)=CC(O[C@H]8[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O8)=CC5

Storage & Solubility Information

Storage

keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc