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PFB-FDG
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Catalog No. T208388Cas No. 209540-64-7
PFB-FDG is a non-fluorescent galactosidase substrate that can be hydrolyzed into the green fluorescent PFB-F (Ex=485 nm, Em=535 nm). It is used for measuring β-galactosidase activity.
PFB-FDG
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Catalog No. T208388Cas No. 209540-64-7
PFB-FDG is a non-fluorescent galactosidase substrate that can be hydrolyzed into the green fluorescent PFB-F (Ex=485 nm, Em=535 nm). It is used for measuring β-galactosidase activity.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 10 mg | Inquiry | Inquiry | Inquiry | |
| 50 mg | Inquiry | Inquiry | Inquiry |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Chemical Properties
| Description | PFB-FDG is a non-fluorescent galactosidase substrate that can be hydrolyzed into the green fluorescent PFB-F (Ex=485 nm, Em=535 nm). It is used for measuring β-galactosidase activity. |
| In vitro | Measurement of β-galactosidase activity involves incubating 2 × 10^5 cells with 50 μg/mL of PFB-FDG in serum-free DMEM containing 25 mM Hepes (pH 7.4) at 37℃ for 2 hours. (Mononuclear cells, for example, may require different concentrations.) Wash the cells once with ice-cold PBS and analyze using flow cytometry (Ex=485 nm, Em=535 nm). [Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).] |
| Molecular Weight | 865.66 |
| Formula | C39H32F5NO16 |
| Cas No. | 209540-64-7 |
| Smiles | O=C1OC2(C=3C1=CC(NC(=O)C4=C(F)C(F)=C(F)C(F)=C4F)=CC3)C=5C(OC=6C2=CC=C(O[C@H]7[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O7)C6)=CC(O[C@H]8[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O8)=CC5 |
Storage & Solubility Information
| Storage | keep away from direct sunlight | store at -20°C | Shipping with blue ice/Shipping at ambient temperature. |
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In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . A total of 10 animals were administered, and the formula you used is 5% 
DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH2O. So your working solution concentration is 2 mg/mL。Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.Preparation method for in vivo formula: Take 50 μL DMSO main solution, add 300 μLPEG300 mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2O mix well and clarify
main solution, add 300 μLPEG300 mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2O mix well and clarifyFor Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.
Dose Conversion
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Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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