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p-Nitrophenylphosphorylcholine

Name: p-Nitrophenylphosphorylcholine
Brand: TargetMol
SKU: T37434
Price: 37 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T37434Cas No. 21064-69-7

Alias p-Nitrophenyl phosphorylcholine, O-(4-Nitrophenylphosphoryl)choline, 4-Nitrophenylphosphorylcholine

p-Nitrophenylphosphorylcholine is a chromogenic substrate for measuring phospholipase C activity, releasing p-nitrophenol that can be quantified at 405 nm.

p-Nitrophenylphosphorylcholine

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Purity: 99.34%

Catalog No. T37434Alias p-Nitrophenyl phosphorylcholine, O-(4-Nitrophenylphosphoryl)choline, 4-NitrophenylphosphorylcholineCas No. 21064-69-7

p-Nitrophenylphosphorylcholine is a chromogenic substrate for measuring phospholipase C activity, releasing p-nitrophenol that can be quantified at 405 nm.

Pack Size	Price	USA Warehouse	Global Warehouse
25 mg	$37	-	In Stock
50 mg	$60	-	In Stock
100 mg	$98	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.34%

Appearance:Solid

Color:White

Resource Download

COA LCMS HNMR

Product Introduction

Bioactivity

Description	p-Nitrophenylphosphorylcholine is a chromogenic substrate for measuring phospholipase C activity, releasing p-nitrophenol that can be quantified at 405 nm.
Synonyms	p-Nitrophenyl phosphorylcholine, O-(4-Nitrophenylphosphoryl)choline, 4-Nitrophenylphosphorylcholine

Chemical Properties

Molecular Weight	304.24
Formula	C₁₁H₁₇N₂O₆P
Cas No.	21064-69-7
Smiles	[O-][N+](C1=CC=C(C=C1)OP([O-])(OCC[N+](C)(C)C)=O)=O

Storage & Solubility Information

Storage

keep away from direct sunlight,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

PBS (pH 7.2): 10 mg/mL (32.87 mM), Sonication is recommended.

DMF: 1 mg/mL (3.29 mM), Sonication is recommended.

DMSO: 20 mg/mL (65.74 mM), Sonication is recommeded.

Ethanol: 10 mg/mL (32.87 mM), Sonication is recommended.

Solution Preparation Table

DMF/PBS (pH 7.2)/Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	3.2869 mL	16.4344 mL	32.8688 mL	164.3439 mL

PBS (pH 7.2)/Ethanol/DMSO

	1mg	5mg	10mg	50mg
5 mM	0.6574 mL	3.2869 mL	6.5738 mL	32.8688 mL
10 mM	0.3287 mL	1.6434 mL	3.2869 mL	16.4344 mL
20 mM	0.1643 mL	0.8217 mL	1.6434 mL	8.2172 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

p-NitrophenylphosphorylcholinepNitrophenylphosphorylcholinep Nitrophenylphosphorylcholine

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