Iron(II) sulfate heptahydrate is an important iron salt commonly used in water treatment as a flocculant, as well as a fertiliser and soil conditioner, and serves as a raw material for the preparation of other iron compounds.

Methods: Iron(II) sulfate heptahydrate (16-125 μM, 48 h) was used to treat K562 chronic myeloid leukemia cells and T47D breast cancer cells to observe the effect on cell growth.
Results: Iron(II) sulfate heptahydrate inhibited the growth of both cell lines. [1]

Methods: Forty male albino rats were divided into two groups: a control group and an anemia group that received a standard iron-free basal diet for 6 weeks. The anemia group was divided into three groups: an anemia control group, an Iron(II) sulfate heptahydrate group (receiving Iron(II) sulfate heptahydrate, 0.4 mg/kg, 10 days) and an iron oxide nanoparticle group (receiving iron oxide nanoparticles 0.4 mg/kg, 10 days) in drinking water to observe the therapeutic effects of Iron(II) sulfate heptahydrate and iron oxide nanoparticles on iron deficiency anemia in rats.
Results: Iron(II) sulfate heptahydrate was not as effective as iron oxide nanoparticles in treating iron deficiency anemia in rats. [2]