IL-4 Protein, Canine, Recombinant

Name: IL-4 Protein, Canine, Recombinant
Brand: TargetMol
SKU: TMPY-02790
Rating: 4.5 (1 reviews)

(Synonyms: interleukin 4) Copy Product Info

Synonyms: interleukin 4

Catalog No. TMPY-02790 Copy Product Info

IL-4 Protein, Canine, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 13 kDa and the accession number is O77762.

Pack Size	Price	USA Stock	Global Stock
5 µg	$129	7-10 days	7-10 days
10 µg	$215	7-10 days	7-10 days
20 µg	$357	7-10 days	7-10 days
50 µg	$519	7-10 days	7-10 days
100 µg	$790	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 5-30 ng/ml.
Description	IL-4 Protein, Canine, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 13 kDa and the accession number is O77762.
Species	Canine
Expression System	E. coli
Tag	Tag Free
Accession Number	O77762
Construction	A DNA sequence encoding the canine IL4 (O77762) (His 25-His 132) was expressed, with an initial Met at the N-terminus. Predicted N terminal: Met
Protein Purity	> 96 % as determined by SDS-PAGE
Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	interleukin 4
Research Background	Interleukin-4, also known as IL4, is a secreted protein that belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation. It enhances both secretion and cell surface expression of IgE and IgG1. Interleukin-4 / IL4 also regulates the expression of the low-affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Interleukin-4 is essential for the switching of B cells to IgE antibody production and the maturation of T helper (Th) cells toward the Th2 phenotype. It participates in at least several B-cell activation processes as well as other cell types. However, studies show that double mutant (Q116D, Y119D) of the murine IL4 protein (QY), both glutamine 116 and tyrosine 119, which binds to the IL4 receptor alpha, completely inhibits in a dose-dependent manner the IL4-induced proliferation of lipopolysaccharide-stimulated murine splenic B-cells, of the murine T cell line CTLL-2, and the murine pre-B-cell line BA/F3. QY also inhibited the IL4-stimulated up-regulation of CD23 expression by lipopolysaccharide-stimulated murine splenic B-cells and abolished tyrosine phosphorylation of the transcription factor Stat6 and the tyrosine kinase Jak3 in IL4-stimulated BA/F3 cells.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

Chemical Properties

Molecular Weight

13 kDa (predicted); 12-14 kDa (reducing conditions)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.