IFNAR1 Protein, Mouse, Recombinant (His)

Name: IFNAR1 Protein, Mouse, Recombinant (His)
Brand: TargetMol
SKU: TMPY-01489
Rating: 4.5 (1 reviews)

(Synonyms: interferon (α, β and ω) receptor 1, interferon (alpha, beta and omega) receptor 1, Infar, Ifrc, Ifnar, Ifar, CD118) Copy Product Info

Synonyms: interferon (α, β and ω) receptor 1, interferon (alpha, beta and omega) receptor 1, Infar, Ifrc, Ifnar, Ifar, CD118

Catalog No. TMPY-01489 Copy Product Info

IFNAR1 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 47 kDa and the accession number is P33896.

Pack Size	Price	USA Stock	Global Stock
5 µg	$68	7-10 days	7-10 days
10 µg	$108	7-10 days	7-10 days
20 µg	$178	7-10 days	7-10 days
50 µg	$359	7-10 days	7-10 days
100 µg	$696	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Measured by its ability to inhibit mIFNB1-mediated protection of L929 mouse fibroblast cells infected with vesicular stomatitis virus (VSV) to viral lysis. The ED50 for this effect is typically 0.2-1μg/mL.
Description	IFNAR1 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 47 kDa and the accession number is P33896.
Species	Mouse
Expression System	HEK293 Cells
Tag	C-His
Accession Number	P33896
Construction	A DNA sequence encoding the extracellular domain of mouse IFNAR1 (NP_034638.2) extracellular domain (Met 1-Thr 429) was expressed, with a polyhistidine tag at the C-terminus. Predicted N terminal: Glu 27
Protein Purity	> 96 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	interferon (α, β and ω) receptor 1, interferon (alpha, beta and omega) receptor 1, Infar, Ifrc, Ifnar, Ifar, CD118
Research Background	Interferon-alpha/beta receptor alpha chain (IFNAR1) is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulate Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. The encoded protein also functions as an antiviral factor. Tyk2 slows down IFNAR1 degradation and that this is due, at least in part, to inhibition of IFNAR1 endocytosis. Mutant versions of IFNAR1, in which Tyr466 is changed to phenylalanine, can act in a dominant-negative manner to inhibit phosphorylation of STAT2. These observations are consistent with a model in which IFNAR1 mediates the interaction between JAK kinases and the STAT transcription factors.

Chemical Properties

Molecular Weight

47 kDa (predicted); 55-60 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.