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HiBiT tag acetate is a complementary peptide (VSGWRLFKKIS) with high affinity for LgBiT. It fuses with the Gβ subunit of the receptor. The complementation of LgBiT and HiBiT enhances the stability of ETR and G proteins. HiBiT can attach to target proteins (GFP) to quantify transport to the cytosol.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 10 mg | Inquiry | Inquiry | Inquiry | |
| 50 mg | Inquiry | Inquiry | Inquiry |
| Description | HiBiT tag acetate is a complementary peptide (VSGWRLFKKIS) with high affinity for LgBiT. It fuses with the Gβ subunit of the receptor. The complementation of LgBiT and HiBiT enhances the stability of ETR and G proteins. HiBiT can attach to target proteins (GFP) to quantify transport to the cytosol. |
| Sequence | Val-Ser-Gly-Trp-Arg-Leu-Phe-Lys-Lys-Ile-Ser |
| Sequence Short | VSGWRLFKKIS |
| Storage | keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
Dissolve 2 mg of the compound in 100 μL DMSO
to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO
stock solution to 400 μL PEG300
and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O
and mix thoroughly until a homogeneous solution is obtained.
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