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HEPES Sodium

Name: HEPES Sodium
Brand: TargetMol
SKU: T22839
Price: 29 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T22839Cas No. 75277-39-3

HEPES Sodium salt is a nonvolatile zwitterionic chemical buffering agent. HEPES Sodium salt is broadly applied in cell culture. HEPES Sodium salt is effective at pH 6.8 to 8.2. HEPES Sodium salt also induces of lysosome biogenesis.

HEPES Sodium

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Purity: 99.91%

Catalog No. T22839Cas No. 75277-39-3

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
1 g	$29	-	In Stock

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All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.91%

Appearance:Solid

Color:White

Resource Download

COA HNMR LCMS

Product Introduction

Bioactivity

Description

In vitro

HEPES Sodium salt maintains superhydrophilicity of titanium for at least 3 months, leading to a continuous retention of bioactivity and osteoconductivity[1]. In cultured RAW264.7 cells, HEPES Sodium salt drives lysosome biogenesis, affects MiT/TFE cytoplasmic-nuclear distribution, disrupts global cellular transcriptional profiles, leading to the activation of a MiT/TFE-dependent lysosomal-autophagic gene network[3].

Chemical Properties

Molecular Weight	260.29
Formula	C₈H₁₇N₂NaO₄S
Cas No.	75277-39-3
Smiles	C1CN(CCN1CCO)CCS(=O)(=O)[O-].[Na+]
Relative Density.	1.504 g/cm3 at 20℃

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: Soluble

DMSO: 125 mg/mL (480.23 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 2.5 mg/mL (9.6 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.8419 mL	19.2093 mL	38.4187 mL	192.0934 mL
5 mM	0.7684 mL	3.8419 mL	7.6837 mL	38.4187 mL
10 mM	0.3842 mL	1.9209 mL	3.8419 mL	19.2093 mL
20 mM	0.1921 mL	0.9605 mL	1.9209 mL	9.6047 mL
50 mM	0.0768 mL	0.3842 mL	0.7684 mL	3.8419 mL
100 mM	0.0384 mL	0.1921 mL	0.3842 mL	1.9209 mL

Calculator

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

HEPES Sodium saltHEPES Sodium