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GSHtracer is a ratiometric fluorescent probe specifically designed for quantitative measurement of intracellular glutathione concentrations, exhibiting a distinct excitation–emission shift from 520/580 nm to 430/510 nm upon GSH binding, GSHtracer is widely used in redox biology research to monitor cellular oxidative stress, antioxidant capacity, and glutathione-dependent signaling pathways.


| Description | GSHtracer is a ratiometric fluorescent probe specifically designed for quantitative measurement of intracellular glutathione concentrations, exhibiting a distinct excitation–emission shift from 520/580 nm to 430/510 nm upon GSH binding, GSHtracer is widely used in redox biology research to monitor cellular oxidative stress, antioxidant capacity, and glutathione-dependent signaling pathways. |
| Molecular Weight | 363.42 |
| Formula | C21H21N3O3 |
| Cas No. | 1479071-34-5 |
| Smiles | N#CC(=CC1=CC=2C=C3C4=C(C2OC1=O)CCCN4CCC3)C(=O)N(C)C |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
Dissolve 2 mg of the compound in 100 μL DMSO
to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO
stock solution to 400 μL PEG300
and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O
and mix thoroughly until a homogeneous solution is obtained.
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