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Fura-2 pentasodium is a ratiometric fluorescent dye utilized for intracellular calcium ion imaging, with a Kd value of 140 nM. Its emission wavelength is 510 nm, and it features excitation wavelengths of 340 nm and 380 nm. The fluorescence intensity ratio of 340/380 is directly proportional to the intracellular Ca2+ (calcium ion) levels.
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| 10 mg | Inquiry | Inquiry | Inquiry | |
| 50 mg | Inquiry | Inquiry | Inquiry |
| Description | Fura-2 pentasodium is a ratiometric fluorescent dye utilized for intracellular calcium ion imaging, with a Kd value of 140 nM. Its emission wavelength is 510 nm, and it features excitation wavelengths of 340 nm and 380 nm. The fluorescence intensity ratio of 340/380 is directly proportional to the intracellular Ca2+ (calcium ion) levels. |
| In vitro | Fura-2 (10 μM, 2 min) pentasodium is utilized for detecting calcium ion concentration fluctuations in heparinized peripheral blood neutrophils. Fura-2 pentapotassium (10 μM, 5 min) serves as a calcium probe to assess the distribution of calcium ions in cardiac mitochondria without affecting its activity in rats. |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
Dissolve 2 mg of the compound in 100 μL DMSO
to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO
stock solution to 400 μL PEG300
and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O
and mix thoroughly until a homogeneous solution is obtained.
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