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FM4-64

🥰Excellent
Catalog No. T23409Cas No. 162112-35-8
Alias SynaptoRedTM C2

FM4-64 (SynaptoRedTM C2) is a styryl dye that stains stably but does not fluoresce in aqueous solution.FM4-64 does not passively diffuse into the plasma membrane bilayer and must be actively transported across the membrane.FM4-64 can be used to study cytotoxicity, cytosolization, and vesicular transport.Cephalexin monohydroxylin monohydroxylin (Cephalexin monohydroxylin) can be used to study cytotoxicity and vesicular transport.

FM4-64

FM4-64

🥰Excellent
Purity: 99.41%
Catalog No. T23409Alias SynaptoRedTM C2Cas No. 162112-35-8
FM4-64 (SynaptoRedTM C2) is a styryl dye that stains stably but does not fluoresce in aqueous solution.FM4-64 does not passively diffuse into the plasma membrane bilayer and must be actively transported across the membrane.FM4-64 can be used to study cytotoxicity, cytosolization, and vesicular transport.Cephalexin monohydroxylin monohydroxylin (Cephalexin monohydroxylin) can be used to study cytotoxicity and vesicular transport.
Pack SizePriceAvailabilityQuantity
1 mg$337In Stock
5 mg$591In Stock
10 mg$857In Stock
25 mg$1,270In Stock
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Purity:99.41%
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Product Introduction

Bioactivity
Description
FM4-64 (SynaptoRedTM C2) is a styryl dye that stains stably but does not fluoresce in aqueous solution.FM4-64 does not passively diffuse into the plasma membrane bilayer and must be actively transported across the membrane.FM4-64 can be used to study cytotoxicity, cytosolization, and vesicular transport.Cephalexin monohydroxylin monohydroxylin (Cephalexin monohydroxylin) can be used to study cytotoxicity and vesicular transport.
Cell Research
Instructions for use
I. Solution preparation
1. Stock solution: Dissolve FM4-64 in DMSO or sterile water to prepare a high concentration stock solution (usually 1–5 mM).
2. Working solution: Dilute the stock solution to the working concentration (usually 1–10 µM) according to the experimental needs, using an appropriate buffer (such as PBS or serum-free medium).
Notes:
1) FM4-64 is a membrane-affinity dye, but it cannot penetrate into cells, so it is suitable for labeling cell membranes or vesicle membranes.
2) The dye will degrade under light, so try to avoid light during operation.
II. Operation steps
1. Cell experiment
1) Cell preparation: Culture cells to an appropriate density. Wash cells with serum-free medium or PBS before staining to remove residual substances.
Staining steps:
2) Add the working solution directly to the cell culture medium and mix gently.
3) Incubate at an appropriate temperature (e.g., 37°C) for 5–30 minutes, which needs to be optimized based on the experiment.
4) Washing: Wash the cells multiple times with an appropriate buffer (e.g., PBS) to remove unbound dye. Optionally, use cold PBS to terminate staining and reduce background signal.
5) Imaging: Observe the sample using a fluorescence microscope.
Excitation wavelength: ~515–540 nm.
Emission wavelength: ~640–700 nm (red fluorescence signal).
2. Tissue experiments
1) Tissue preparation: Fix and permeabilize tissue sections (if necessary) to enhance dye penetration.
2) Staining steps: Add working solution to tissue sections and incubate for 10–30 minutes.
3) Washing: Wash tissue sections with PBS to remove excess dye.
4) Imaging: Also observe the red fluorescence signal using a fluorescence microscope.
AliasSynaptoRedTM C2
Chemical Properties
Molecular Weight607.51
FormulaC30H45Br2N3
Cas No.162112-35-8
Smiles[Br-].[Br-].CCN(CC)c1ccc(\C=C/C=C/C=C\c2cc[n+](CCC[N+](CC)(CC)CC)cc2)cc1
Relative Density.no data available
Storage & Solubility Information
Storagekeep away from moisture,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
DMSO: 30 mg/mL (49.38 mM), Sonication is recommended.
Solution Preparation Table
DMSO
1mg5mg10mg50mg
1 mM1.6461 mL8.2303 mL16.4606 mL82.3032 mL
5 mM0.3292 mL1.6461 mL3.2921 mL16.4606 mL
10 mM0.1646 mL0.8230 mL1.6461 mL8.2303 mL
20 mM0.0823 mL0.4115 mL0.8230 mL4.1152 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
TargetMol | Animal experimentsFor example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH2O. So your working solution concentration is 2 mg/mL。
Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSOTargetMol | reagent (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2OTargetMol | reagent mix well and clarify
For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.
1 Enter information below:
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2 Enter the in vivo formulation:
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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