Estradiol/OVA is a versatile dye, serving as a crucial tool in biological experiments. It aids researchers in observing and analyzing cellular structures, tracking biomolecules, assessing cell functions, distinguishing cell types, detecting biomolecules, studying tissue pathology, and monitoring microorganisms. Its applications range from fundamental scientific research to clinical diagnostics. This dye is extensively used in traditional fields such as textile dyeing, as well as in emerging areas like functional textile treatment, food coloring, and dye-sensitized solar cells.