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Di-8-ANEPPS

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Catalog No. T18973Cas No. 157134-53-7

Di-8-ANEPPS is a voltage-sensitive dye and fast-response probe that fluoresces in response to potential changes in the environment and is capable of detecting transient potential changes in cells, with a 2-10% fluorescence change per 100 mV and potential-dependent changes in the excitation spectrum.

Di-8-ANEPPS

Di-8-ANEPPS

🥰Excellent
Catalog No. T18973Cas No. 157134-53-7
Di-8-ANEPPS is a voltage-sensitive dye and fast-response probe that fluoresces in response to potential changes in the environment and is capable of detecting transient potential changes in cells, with a 2-10% fluorescence change per 100 mV and potential-dependent changes in the excitation spectrum.
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1 mg$116In Stock
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Product Introduction

Bioactivity
Description
Di-8-ANEPPS is a voltage-sensitive dye and fast-response probe that fluoresces in response to potential changes in the environment and is capable of detecting transient potential changes in cells, with a 2-10% fluorescence change per 100 mV and potential-dependent changes in the excitation spectrum.
In vitro
Di-8-ANEPPS (5 μM) was applied to mouse ventricular myocardium for 20 minutes and used to record action potential propagation. Di-8-ANEPPS reduced gap junctional conductance and slowed conduction velocity by lowering phosphorylation of Connexin43[1].
Chemical Properties
Molecular Weight592.88
FormulaC36H52N2O3S
Cas No.157134-53-7
SmilesO=S(=O)([O-])CCC[N+]1=CC=C(C=CC=2C=CC3=CC(=CC=C3C2)N(CCCCCCCC)CCCCCCCC)C=C1
Relative Density.1.31g/cm3
Storage & Solubility Information
Storagekeep away from direct sunlight | store at -20°C | Shipping with blue ice/Shipping at ambient temperature.
Solubility Information
DMSO: 1 mg/mL (1.69 mM), Sonication is recommended.
Solution Preparation Table
DMSO
1mg5mg10mg50mg
1 mM1.6867 mL8.4334 mL16.8668 mL84.3341 mL

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TargetMol | Animal experimentsFor example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH2O. So your working solution concentration is 2 mg/mL。
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Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2OTargetMol | reagent mix well and clarify
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Keywords

Di-8-ANEPPSDi8ANEPPSDi 8 ANEPPS
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