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Di-8-ANEPPS
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Catalog No. T18973Cas No. 157134-53-7
Di-8-ANEPPS is a voltage-sensitive dye and fast-response probe that fluoresces in response to potential changes in the environment and is capable of detecting transient potential changes in cells, with a 2-10% fluorescence change per 100 mV and potential-dependent changes in the excitation spectrum.
Di-8-ANEPPS
😃Good
Catalog No. T18973Cas No. 157134-53-7
Di-8-ANEPPS is a voltage-sensitive dye and fast-response probe that fluoresces in response to potential changes in the environment and is capable of detecting transient potential changes in cells, with a 2-10% fluorescence change per 100 mV and potential-dependent changes in the excitation spectrum.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 1 mg | $99 | - | In Stock |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Appearance:Solid
Color:White to Red
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Chemical Properties
Storage & Solubility Information
| Description | Di-8-ANEPPS is a voltage-sensitive dye and fast-response probe that fluoresces in response to potential changes in the environment and is capable of detecting transient potential changes in cells, with a 2-10% fluorescence change per 100 mV and potential-dependent changes in the excitation spectrum. |
| In vitro | Di-8-ANEPPS (5 μM) was applied to mouse ventricular myocardium for 20 minutes and used to record action potential propagation. Di-8-ANEPPS reduced gap junctional conductance and slowed conduction velocity by lowering phosphorylation of Connexin43[1]. |
| Molecular Weight | 592.88 |
| Formula | C36H52N2O3S |
| Cas No. | 157134-53-7 |
| Smiles | O=S(=O)([O-])CCC[N+]1=CC=C(C=CC=2C=CC3=CC(=CC=C3C2)N(CCCCCCCC)CCCCCCCC)C=C1 |
| Relative Density. | 1.31g/cm3 |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | Shipping with blue ice/Shipping at ambient temperature. | ||||||||||
| Solubility Information | DMSO: 1 mg/mL (1.69 mM), Sonication is recommended. | ||||||||||
Solution Preparation Table | |||||||||||
DMSO
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Calculator
In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Stock Solution Preparation:
Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
Preparation of the In Vivo Formulation:
1) Add 100 μL of the DMSO stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
Dose Conversion
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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