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DFHBI-BI
Catalog No. T217346 Copy Product Info
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DFHBI-BI is an analog of the green fluorescent protein chromophore. It can bind to Broccoli (a fluorescent RNA aptamer) and is activated by this interaction, exhibiting higher binding affinity. In mammalian cells, DFHBI-BI prevents thermal denaturation of Broccoli at 37°C, thereby increasing the formation of Broccoli-BI complexes with fluorescent properties. Its fluorescence wavelength is 559/635 nm.
DFHBI-BI
Copy Product Info🥰Excellent
Catalog No. T217346
DFHBI-BI is an analog of the green fluorescent protein chromophore. It can bind to Broccoli (a fluorescent RNA aptamer) and is activated by this interaction, exhibiting higher binding affinity. In mammalian cells, DFHBI-BI prevents thermal denaturation of Broccoli at 37°C, thereby increasing the formation of Broccoli-BI complexes with fluorescent properties. Its fluorescence wavelength is 559/635 nm.
DFHBI-BI
Cas No. 2561482-42-4
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 10 mg | Inquiry | Inquiry | Inquiry | |
| 50 mg | Inquiry | Inquiry | Inquiry |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Description | DFHBI-BI is an analog of the green fluorescent protein chromophore. It can bind to Broccoli (a fluorescent RNA aptamer) and is activated by this interaction, exhibiting higher binding affinity. In mammalian cells, DFHBI-BI prevents thermal denaturation of Broccoli at 37°C, thereby increasing the formation of Broccoli-BI complexes with fluorescent properties. Its fluorescence wavelength is 559/635 nm. |
| In vitro | DFHBI-BI (10 μM) dye results in low fluorescence intensity after a 2-hour staining of cells. However, when DFHBI-BI is applied to HEK293T cells expressing Broccoli circular RNA, an increase in fluorescence intensity is observed. |
| Molecular Weight | 368.34 |
| Formula | C19H14F2N4O2 |
| Cas No. | 2561482-42-4 |
| Smiles | C(C1=C2C(=CC=C1)N=CN2)N3C(=O)\C(=C\C4=CC(F)=C(O)C(F)=C4)\N=C3C |
| Storage | Store at -20°C Shipping with blue ice/Shipping at ambient temperature. |
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In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Stock Solution Preparation:
Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
Preparation of the In Vivo Formulation:
1) Add 100 μL of the DMSO stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
Dose Conversion
You can also refer to dose conversion for different animals. More Dose Conversion
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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