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DAz-2

Name: DAz-2
Brand: TargetMol
SKU: T86163
Rating: 4.5 (1 reviews)

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Catalog No. T86163Cas No. 1176905-54-6

Alias DCP-N3

DAz-2 is a cell-permeable sulfonic acid probe that can directly detect sulfonic acid-modified proteins in living cells [1].

DAz-2

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Catalog No. T86163Alias DCP-N3Cas No. 1176905-54-6

DAz-2 is a cell-permeable sulfonic acid probe that can directly detect sulfonic acid-modified proteins in living cells [1].

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
10 mg	Inquiry	Inquiry	Inquiry
50 mg	Inquiry	Inquiry	Inquiry

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Product Introduction

Bioactivity

Description	DAz-2 is a cell-permeable sulfonic acid probe that can directly detect sulfonic acid-modified proteins in living cells [1].
In vitro	DAz-2 is utilized for detecting sulfonic acids under various conditions [1]: 1. Prepare 10 μM protein, 50 μg cellular lysate, or 200 μg protein lysate. 2. Add 50 or 100 μM H2O2 and incubate at room temperature for 20 minutes; then add 1 mM Daz-2 or 5% (v/v) DMSO, and react at room temperature for 15 minutes. 3. Quench the reaction with 5 mM DTT and remove excess reagents via gel filtration. 4. Separate proteins using 4-12% SDS-PAGE, transfer to a PVDF membrane, and detect using HRP-Streptavidin Western Blotting. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
Synonyms	DCP-N3

Chemical Properties

Molecular Weight	195.22
Formula	C₉H₁₃N₃O₂
Cas No.	1176905-54-6
Smiles	C(CCN=[N+]=[N-])C1C(=O)CC(=O)CC1

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc