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DAz-1
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Catalog No. T86162Cas No. 1112977-84-0
Alias Click Tag™ DAz-1
DAz-1 is a cell-permeable sulfonic acid probe that directly detects sulfonic acid-modified proteins in living cells [1].
DAz-1
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Catalog No. T86162Alias Click Tag™ DAz-1Cas No. 1112977-84-0
DAz-1 is a cell-permeable sulfonic acid probe that directly detects sulfonic acid-modified proteins in living cells [1].
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 mg | $1,520 | 4-6 weeks | 4-6 weeks | |
| 50 mg | $1,980 | 4-6 weeks | 4-6 weeks | |
| 100 mg | $2,500 | 4-6 weeks | 4-6 weeks |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Chemical Properties
| Description | DAz-1 is a cell-permeable sulfonic acid probe that directly detects sulfonic acid-modified proteins in living cells [1]. |
| In vitro | Detection methods for DAz-1 sulfonic acid under various conditions [1]: Prepare a 10 μM protein solution, 50 μg of cell lysate, or 200 μg of protein lysate. Add 50 or 100 μM H2O2 and incubate at room temperature for 20 minutes; then, introduce 1 mM Daz-1 or 5% (v/v) DMSO, continuing the reaction at room temperature for 15 minutes. Quench the reaction using 5 mM DTT, and remove excess reagents via gel filtration. Separate proteins using 4-12% SDS-PAGE, transfer them to a PVDF membrane, and detect with HRP-Streptavidin WB. |
| Synonyms | Click Tag™ DAz-1 |
| Molecular Weight | 238.24 |
| Formula | C10H14N4O3 |
| Cas No. | 1112977-84-0 |
| Smiles | C(NCCCN=[N+]=[N-])(=O)C1CC(=O)CC(=O)C1 |
Storage & Solubility Information
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
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In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . A total of 10 animals were administered, and the formula you used is 5% 
DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH2O. So your working solution concentration is 2 mg/mL。Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.Preparation method for in vivo formula: Take 50 μL DMSO main solution, add 300 μLPEG300 mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2O mix well and clarify
main solution, add 300 μLPEG300 mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2O mix well and clarifyFor Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.
Dose Conversion
You can also refer to dose conversion for different animals. More Dose Conversion
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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