Cy5-N3 triethylamine salt is a turnip-based fluorescent dye.CY5-N3 can be used to study cellular imaging.

1. Solution configuration
1. Mother liquor configuration: Dissolve Cy5-N3 in an appropriate solvent, usually DMSO, and formulate to the desired concentration (10 mM).
2. Working solution configuration: Dilute Cy5-N3 mother liquor with PBS/DMEM/H2O to the desired concentration, usually 1-10 µM.
2. Cell imaging
Experimental steps:
1. Label cells: Add Cy5-N3 solution to the cell culture medium and incubate with the cells for a certain period of time, usually from 30 minutes to 1 hour (adjust according to the specific requirements of the experiment).
2. Rinse: After the incubation is complete, wash the cells through PBS buffer to remove excess dye.
3. Fluorescence microscopy imaging: Use a fluorescence microscope (excitation wavelength is 649 nm and emission wavelength is 670 nm) to observe the Cy5 marker in the cells and record the fluorescence image.
III. Protein or nucleic acid labeling
Experimental steps:
1. Reaction with protein or nucleic acid: Add Cy5-N3 to the protein or nucleic acid solution, the reaction time is usually 1 hour and the temperature is room temperature to ensure a labeling reaction with the target molecule.
2. Purification: Use dialysis or other methods to remove unbound Cy5-N3.
3. Fluorescence detection: Use a fluorescence spectrophotometer or fluorescence microscope to measure and observe the labeling effect.
IV. Cell/tissue staining
Experimental steps:
1. Treat cells or tissues: Add Cy5-N3 to cell or tissue sections and adjust the incubation time according to the experimental needs (usually 30 minutes to 1 hour).
2. Cleaning: Wash with PBS or other buffer to remove excess dye.
3. Fluorescence imaging: Use a fluorescence microscope to observe the staining effect and record the image.
4. Molecular detection
Experimental steps:
1. Labeling reaction: React Cy5-N3 with target molecules (such as DNA, RNA, protein) to ensure that the labeling is completed.
2. Detection and analysis: Use appropriate detection methods (such as fluorescence microscopy, spectrophotometer) to observe the localization, concentration and distribution of labeled molecules.