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CoelPhos
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Catalog No. T219032 Copy Product Info
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CoelPhos is an impermeable cell membrane derivative of coelenterazine. It specifically recognizes extracellular GLuc and emits a luminescent signal. CoelPhos serves as a practical bioluminescent tool for monitoring exocytosis processes, such as cell surface membrane fusion.
CoelPhos
Cas No. 1643661-02-2
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 10 mg | Inquiry | Inquiry | Inquiry | |
| 50 mg | Inquiry | Inquiry | Inquiry |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Description | CoelPhos is an impermeable cell membrane derivative of coelenterazine. It specifically recognizes extracellular GLuc and emits a luminescent signal. CoelPhos serves as a practical bioluminescent tool for monitoring exocytosis processes, such as cell surface membrane fusion. |
| In vitro | CoelPhos generates a detectable bioluminescent signal in HeLa cells expressing GLucM23 located in the outer membrane, with an activity reaching approximately 3-4% of natural coelenterazine. However, CoelPhos is unable to permeate the cell membrane, as evidenced by its inability to produce a bioluminescent signal in HeLa cells expressing GLucER located in the endoplasmic reticulum. |
| Molecular Weight | 656.62 |
| Formula | C33H33N6O7P |
| Cas No. | 1643661-02-2 |
| Smiles | O=C1C(=NC2=C(NC(=CN12)C=3C=CC(O)=CC3)CC=4C=CC=CC4)CC5=CC=C(OCCCN6N=NC(=C6)COCP(=O)(O)O)C=C5 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. |
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In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Stock Solution Preparation:
Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
Preparation of the In Vivo Formulation:
1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 µL Tween 80 and mix well until fully clarified.
3) Add 450 µL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
Dose Conversion
You can also refer to dose conversion for different animals. More Dose Conversion
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: CoelPhos in vitro | CoelPhos formula | CoelPhos molecular weight
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