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CEMT
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Catalog No. T218399 Copy Product Info
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CEMT is a carboxylesterase (CE) substrate and a ratiometric two-photon fluorescent reporter probe. Once hydrolyzed by CE, CEMT generates HMT, which serves as a sensor for mitochondrial pH. Upon activation by CE, CEMT exhibits ratiometric fluorescence changes in response to pH variations. It targets mitochondria and covalently binds to them, preventing leakage during acidification and enabling in situ imaging.
CEMT
Cas No. 2413816-64-3
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 10 mg | Inquiry | Inquiry | Inquiry | |
| 50 mg | Inquiry | Inquiry | Inquiry |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Description | CEMT is a carboxylesterase (CE) substrate and a ratiometric two-photon fluorescent reporter probe. Once hydrolyzed by CE, CEMT generates HMT, which serves as a sensor for mitochondrial pH. Upon activation by CE, CEMT exhibits ratiometric fluorescence changes in response to pH variations. It targets mitochondria and covalently binds to them, preventing leakage during acidification and enabling in situ imaging. |
| In vitro | CEMT (10 μM) in a cell-free 40 mM B-R buffer (pH 7.42, 37 °C) displays a linear ratiometric fluorescence response to carboxylesterase concentrations ranging from 0 to 15 μg/mL; its hydrolysis product, HMT, catalyzed by carboxylesterase (CE), shows a similar fluorescence response to pH values between 4.98 and 9.07. The intracellular hydrolysis product HMT of CEMT (10 μM; 20 min) exhibits strong mitochondrial colocalization in HepG2 cells, with minimal leakage even under acidic conditions (pH 4.98), confirming CEMT’s ability to anchor mitochondria for in situ imaging. |
| Molecular Weight | 482.36 |
| Formula | C26H21Cl2NO4 |
| Cas No. | 2413816-64-3 |
| Smiles | O(C(C)=O)C=1C=C2C(C=C(/C=C/C=3C=C[N+](CC4=CC=C(CCl)C=C4)=CC3)C(=O)O2)=CC1.[Cl-] |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. |
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In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Stock Solution Preparation:
Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
Preparation of the In Vivo Formulation:
1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 µL Tween 80 and mix well until fully clarified.
3) Add 450 µL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
Dose Conversion
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: CEMT in vitro | CEMT formula | CEMT molecular weight
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