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CellTracker Orange CMRA Dye is a fluorescent dye used for cell imaging and tracking cell movement and location.

| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 10 mg | Inquiry | 10-14 weeks | 10-14 weeks | |
| 50 mg | Inquiry | 10-14 weeks | 10-14 weeks |
| Description | CellTracker Orange CMRA Dye is a fluorescent dye used for cell imaging and tracking cell movement and location. |
| In vitro | CellTracker Orange CMRA Dye Cell Viability Assay: Begin by adding 5 μM dye to each sample, maintaining a temperature of 27-33℃ for 30 minutes to label the samples. Next, centrifuge the cells at 1000 g for 10 minutes, then resuspend the cell pellet in pre-warmed 10% iFCS RPMI at a concentration of 3*10^7 cells/mL. Incubate the cells again at 27-33℃ for another 30 minutes. Following this, centrifuge at 1000 g for 10 minutes and wash with pre-warmed PBS without Ca 2+ and Mg 2+. Centrifuge once more at 1000 g for 10 minutes and resuspend the cells in pre-warmed 10% iFCS RPMI. Finally, measure the fluorescence intensity with an excitation of 548 nm and emission of 576 nm. Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands. |
| Molecular Weight | 550.43 |
| Formula | C30H25Cl2NO5 |
| Cas No. | 3031711-06-2 |
| Smiles | O=C1OC2(C3=CC=C(C=C13)CCl)C4=CC(Cl)=C(OC(=O)C)C=C4OC5=CC6=C(C=C52)C(=CC(N6C)(C)C)C |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
Dissolve 2 mg of the compound in 100 μL DMSO
to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO
stock solution to 400 μL PEG300
and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O
and mix thoroughly until a homogeneous solution is obtained.
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