Carboxymethyl chitosan

Carboxymethyl chitosan is a biochemical reagent used as a biomaterial for life science research and as a sulfonylation reagent for organic synthesis and drug discovery. Carboxymethyl chitosan is applied in biomaterials research focusing on biopolymer modification, drug delivery system modeling, and chemical derivatization strategies in medicinal chemistry-oriented experimental systems.

Methods：Different concentrations of carboxymethyl chitosan were applied to skin fibroblasts, Schwann cells, human umbilical vein endothelial cells, chondrocytes and various tumor cells. Cellular proliferation, migration, viability and apoptosis were observed.
Results：
1.Treatment at 100 μg/mL for 4 days promoted proliferation of normal skin fibroblasts and inhibited proliferation of keloid fibroblasts [1].
2.Carboxymethyl chitosan hydrogel exhibited superior pro-migration effect compared with ordinary chitosan hydrogel [2].
3.Treatment with 50~200 μg/mL for 8 h enhanced the viability of oxidatively damaged Schwann cells and reduced LDH release in a dose-dependent manner [3].
4.Intervention with 0.5~2.0 mg/mL for 24~48 h markedly suppressed the migration of human umbilical vein endothelial cells [4].
5.Incubation at 50~200 μg/mL for 1 h dose-dependently alleviated IL-1β-induced apoptosis of rabbit chondrocytes [5].
6.Treatment with 4.0~5.0 mg/mL for 48 h reduced the viability of multiple tumor cell lines [6].
7.Exposure to 1.0~5.0 mg/mL for 12~24 h inhibited hepatocellular carcinoma cell migration in a dose-dependent manner [7].

Methods：Mouse H22 hepatocarcinoma and Lewis tumor models were established. Carboxymethyl chitosan was administrated via intraperitoneal and intravenous injection at different doses and frequencies. Tumor angiogenesis, tumor growth and organ morphology were observed. Changes in blood-related indicators were detected in rats after high-dose administration.
Results：Intraperitoneal injection of carboxymethyl chitosan at 75–300 mg/kg inhibited tumor angiogenesis in H22 tumor model [4]. Intravenous administration at 20–120 mg/kg suppressed the growth and metastasis of Lewis tumor, increased spleen index and improved histological morphology of liver and lung tissues [6]. Intraperitoneal injection of carboxymethyl chitosan at 1350 mg/kg exerted no adverse effects on blood coagulation, fibrinolysis and hemorheological parameters in rats [8].

H2O: 8 mg/mL, Sonication is recommended.