HGFR/c-Met Protein, Human, Recombinant (hFc)

Name: HGFR/c-Met Protein, Human, Recombinant (hFc)
Brand: TargetMol
SKU: TMPY-05441
Rating: 4.5 (1 reviews)

(Synonyms: RCCP2, MET proto-oncogene, receptor tyrosine kinase, HGFR, DFNB97, c-Met, AUTS9) Copy Product Info

Synonyms: RCCP2, MET proto-oncogene, receptor tyrosine kinase, HGFR, DFNB97, c-Met, AUTS9

Catalog No. TMPY-05441 Copy Product Info

HGFR/c-Met Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The accession number is P08581-1.

Pack Size	Price	USA Stock	Global Stock
5 µg	$58	7-10 days	7-10 days
10 µg	$93	7-10 days	7-10 days
20 µg	$152	7-10 days	7-10 days
50 µg	$298	7-10 days	7-10 days
100 µg	$576	7-10 days	7-10 days
200 µg	$819	7-10 days	7-10 days
500 µg	$1,550	7-10 days	7-10 days
1 mg	$2,980	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Immobilized Human HGF, His Tag at 2 μg/ml (100 μl/Well) on the plate. Dose response curve for Human HGF R, hFc Tag with the EC50 of 9.9 ng/ml determined by ELISA (QC Test). Immobilized Human HGF R, hFc Tag at 5 μg/ml (100 μl/Well) on the plate. Dose response curve for Biotinylated Anti-HGF R Antibody, hFc Tag with the EC50 of 4.2 ng/ml determined by ELISA. Loaded Human HGF R, hFc Tag on ProA-Biosensor can bind Human HGF, His Tag with an affinity constant of 4.32 nM as determined in BLI assay.
Description	HGFR/c-Met Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The accession number is P08581-1.
Species	Human
Expression System	HEK293 Cells
Tag	C-hFc
Accession Number	P08581-1
Construction	Glu25-Thr932
Protein Purity	> 95% as determined by Bis-Tris PAGE; > 90% as determined by HPLC
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonyms	RCCP2, MET proto-oncogene, receptor tyrosine kinase, HGFR, DFNB97, c-Met, AUTS9
Research Background	c-Met, also called tyrosine-protein kinase Met or hepatocyte growth factor receptor (HGF R), is a protein that in humans is encoded by the MET gene. The protein possesses tyrosine kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling.

Chemical Properties

Molecular Weight

The mature form of HGF R is a heterodimer which can be cleaved into α and β chain. The protein has a predicted MW of 32.5 kDa (α chain) and 95.9 kDa (β chain Fc chimera). Due to glycosylation, the protein migrates to 45-55 kDa and 100-120 kDa based on Bis-Tris PAGE result.

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.