HGFR/c-Met Protein, Canine, Recombinant (His)

Name: HGFR/c-Met Protein, Canine, Recombinant (His)
Brand: TargetMol
SKU: TMPY-02068
Rating: 4.5 (1 reviews)

(Synonyms: MET proto-oncogene, receptor tyrosine kinase) Copy Product Info

Synonyms: MET proto-oncogene, receptor tyrosine kinase

Catalog No. TMPY-02068 Copy Product Info

HGFR/c-Met Protein, Canine, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 103 kDa and the accession number is Q75ZY9.

Pack Size	Price	USA Stock	Global Stock
5 µg	$68	7-10 days	7-10 days
10 µg	$108	7-10 days	7-10 days
20 µg	$178	7-10 days	7-10 days
50 µg	$359	7-10 days	7-10 days
100 µg	$696	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	1. Measured by its binding ability in a functional ELISA. 2. Immobilized human HGF at 10 μg/mL (100 μl/well) can bind canine c-MET , The EC50 of canine c-MET is 7 ng/mL.
Description	HGFR/c-Met Protein, Canine, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 103 kDa and the accession number is Q75ZY9.
Species	Canine
Expression System	HEK293 Cells
Tag	N-His
Accession Number	Q75ZY9
Construction	A DNA sequence encoding the extracellular domain of canine MET (NP_001002963.1) (Met 1-Leu 935) was expressed, with a polyhistidine tag at the N-terminus. Predicted N terminal: Glu 25
Protein Purity	> 95 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	MET proto-oncogene, receptor tyrosine kinase
Research Background	Hepatocyte growth factor receptor (HGFR), also known as c-Met or mesenchymal-epithelial transition factor (MET), is a receptor tyrosine kinase (RTK) that is overexpressed and/or mutated in a variety of malignancies. HGFR protein is produced as a single-chain precursor, and HGF is the only known ligand. Normal HGF/HGFR signaling is essential for embryonic development, tissue repair, or wound healing, whereas aberrantly active HGFR has been strongly implicated in tumorigenesis, particularly in the development of invasive and metastatic phenotypes. HGFR protein is a multifaceted regulator of growth, motility, and invasion, and is normally expressed by cells of epithelial origin. Preclinical studies suggest that targeting aberrant HGFR signaling could be an attractive therapy in cancer.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

Chemical Properties

Molecular Weight

103 kDa (predicted); 42-47 kDa and 85-95 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.