Orange I (α-Naphthol Orange) is an aromatic sulfonated p-azo dye that hinders microbial degradation.

Instructions
1. Solvent selection:
Orange I is a water-soluble dye that is usually dissolved in deionized or distilled water.
It has a high solubility and is usually used in a concentration of 0.01% to 0.1% (w/v).
2. Electrophoresis tracer:
Orange I is commonly used as a tracer dye in gel electrophoresis and can be used to monitor the migration process of samples, especially the separation of proteins and nucleic acids.
Instructions for use: Mix an appropriate amount of Orange I solution with the sample and add it to the gel. It can also be added to the electrophoresis buffer before electrophoresis.
3. pH indicator:
As an acid-base indicator, Orange I can change color in the pH range of 2 to 4. It is orange at low pH and yellow at high pH.
Instructions for use: It is suitable for solution pH detection, especially in acidic environments with a small pH range.
4. Staining uses:
In biological experiments, Orange I is used to stain cells, tissue sections or biomolecules. For example, it is used for acid staining of cells or tissue sections.
Usage: Add Orange I to the staining solution, and after staining for a certain period of time, observe the results under a microscope.