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Orange I

Name: Orange I
Brand: TargetMol
SKU: T30663
Price: 31 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T30663Cas No. 523-44-4

Alias α-Naphthol Orange, Tertracid Orange I, Orange I, sodium salt, C.I. Acid Orange 20

Orange I (α-Naphthol Orange) is an aromatic sulfonated p-azo dye that hinders microbial degradation.

Orange I

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Purity: 96.48%

Catalog No. T30663Alias α-Naphthol Orange, Tertracid Orange I, Orange I, sodium salt, C.I. Acid Orange 20Cas No. 523-44-4

Orange I (α-Naphthol Orange) is an aromatic sulfonated p-azo dye that hinders microbial degradation.

Pack Size	Price	USA Warehouse	Global Warehouse
500 mg	$31	In Stock	In Stock
1 g	$41	In Stock	In Stock
5 g	$96	7-10 days	7-10 days
10 g	$139	7-10 days	7-10 days

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:96.48%

Appearance:Solid

Color:Red

Resource Download

COA HNMR HPLC

Product Introduction

Bioactivity

Description	Orange I (α-Naphthol Orange) is an aromatic sulfonated p-azo dye that hinders microbial degradation.
Cell Research	Instructions 1. Solvent selection: Orange I is a water-soluble dye that is usually dissolved in deionized or distilled water. It has a high solubility and is usually used in a concentration of 0.01% to 0.1% (w/v). 2. Electrophoresis tracer: Orange I is commonly used as a tracer dye in gel electrophoresis and can be used to monitor the migration process of samples, especially the separation of proteins and nucleic acids. Instructions for use: Mix an appropriate amount of Orange I solution with the sample and add it to the gel. It can also be added to the electrophoresis buffer before electrophoresis. 3. pH indicator: As an acid-base indicator, Orange I can change color in the pH range of 2 to 4. It is orange at low pH and yellow at high pH. Instructions for use: It is suitable for solution pH detection, especially in acidic environments with a small pH range. 4. Staining uses: In biological experiments, Orange I is used to stain cells, tissue sections or biomolecules. For example, it is used for acid staining of cells or tissue sections. Usage: Add Orange I to the staining solution, and after staining for a certain period of time, observe the results under a microscope. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
Synonyms	α-Naphthol Orange, Tertracid Orange I, Orange I, sodium salt, C.I. Acid Orange 20

Chemical Properties

Molecular Weight	350.32
Formula	C₁₆H₁₁N₂NaO₄S
Cas No.	523-44-4
Smiles	O=S([O-])(C1=CC=C(C=C1)N=NC2=CC=C(C3=C2C=CC=C3)O)=O.[Na+]
Relative Density.	no data available

Storage & Solubility Information

Storage

keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 35.03 mg/mL (99.99 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.8545 mL	14.2727 mL	28.5453 mL	142.7266 mL
5 mM	0.5709 mL	2.8545 mL	5.7091 mL	28.5453 mL
10 mM	0.2855 mL	1.4273 mL	2.8545 mL	14.2727 mL
20 mM	0.1427 mL	0.7136 mL	1.4273 mL	7.1363 mL
50 mM	0.0571 mL	0.2855 mL	0.5709 mL	2.8545 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc