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Beta-Actin Loading Control Antibody (1W773)

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Catalog No. TMAB-00215
Alias β-Actin Protein, β-actin, Beta-Actin, Actin Cytoplasmic 1, ACTB

Beta-Actin Loading Control Antibody (1W773) is a Mouse antibody targeting Beta-Actin Loading Control. Beta-Actin Loading Control Antibody (1W773) can be used in WB,IHC-P,IHC-Fr,IF.

Beta-Actin Loading Control Antibody (1W773)

Beta-Actin Loading Control Antibody (1W773)

😃Good
Catalog No. TMAB-00215Alias β-Actin Protein, β-actin, Beta-Actin, Actin Cytoplasmic 1, ACTB
Beta-Actin Loading Control Antibody (1W773) is a Mouse antibody targeting Beta-Actin Loading Control. Beta-Actin Loading Control Antibody (1W773) can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$1267-10 days
100 μL$1827-10 days
500 μL$7557-10 days
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Product Introduction

Bioactivity
Description
Beta-Actin Loading Control Antibody (1W773) is a Mouse antibody targeting Beta-Actin Loading Control. Beta-Actin Loading Control Antibody (1W773) can be used in WB,IHC-P,IHC-Fr,IF.
Aliasβ-Actin Protein, β-actin, Beta-Actin, Actin Cytoplasmic 1, ACTB
Ig Type
IgG
Clone
1W773
Reactivity
Human,Mouse,Rat (predicted:Chicken,Dog,Rabbit,Hamster,Monkey)
Verified Activity
1. 25 μg total protein per Lane of various lysates probed with beta-Actin monoclonal antibody, unconjugated (TMAB-00215) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Rat Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Monoclonal Antibody, Unconjugated (TMAB-00215) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Monoclonal Antibody, Unconjugated (ascites of TMAB-00215) at 1:1500 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Monoclonal Antibody, Unconjugated (TMAB-00215) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Monoclonal Antibody, Unconjugated (ascites of TMAB-00215) at 1:1500 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Monoclonal Antibody, Unconjugated (ascites of TMAB-00215) at 1:1500 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Monoclonal Antibody, Unconjugated (ascites of TMAB-00215) at 1:1500 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Monoclonal Antibody, Unconjugated (TMAB-00215) at 1:1000 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green), DAPI (blue) was used to stain the cell nucleus.
9. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Monoclonal Antibody, Unconjugated (TMAB-00215) at 1:1000 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green), DAPI (blue) was used to stain the cell nucleus.
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-5000; IHC-P: 1:200-2000; IHC-Fr: 1:200-2000; IF: 1:200-2000
Antibody Type
Monoclonal
Host SpeciesMouse
Subcellular LocalizationCytoplasm, cytoskeleton.
ConstructionHybridoma Monoclonal Antibody
PurificationProtein G purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundActin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence. Antibodies against plant Actin are useful as loading controls for Western Blotting.
Antigen Details
Immunogen
Synthetic MAP peptide: human beta-Actin
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Actin,Actin,Beta Actin
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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