Beta-Actin Loading Control Antibody (1W773) is a Mouse antibody targeting Beta-Actin Loading Control. Beta-Actin Loading Control Antibody (1W773) can be used in WB,IHC-P,IHC-Fr,IF.

IgG

1W773

Human,Mouse,Rat (predicted:Chicken,Dog,Rabbit,Hamster,Monkey)

1. 25 μg total protein per Lane of various lysates probed with beta-Actin monoclonal antibody, unconjugated (TMAB-00215) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Rat Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Monoclonal Antibody, Unconjugated (TMAB-00215) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Monoclonal Antibody, Unconjugated (ascites of TMAB-00215) at 1:1500 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Monoclonal Antibody, Unconjugated (TMAB-00215) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Monoclonal Antibody, Unconjugated (ascites of TMAB-00215) at 1:1500 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Monoclonal Antibody, Unconjugated (ascites of TMAB-00215) at 1:1500 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Monoclonal Antibody, Unconjugated (ascites of TMAB-00215) at 1:1500 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Monoclonal Antibody, Unconjugated (TMAB-00215) at 1:1000 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green), DAPI (blue) was used to stain the cell nucleus.
9. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Monoclonal Antibody, Unconjugated (TMAB-00215) at 1:1000 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green), DAPI (blue) was used to stain the cell nucleus.

WB: 1:500-5000; IHC-P: 1:200-2000; IHC-Fr: 1:200-2000; IF: 1:200-2000

Monoclonal

Synthetic MAP peptide: human beta-Actin

Human

Actin,Actin,Beta Actin