BD-105

BD-105 is a glucagon-binding fluorescent probe with a Ka value of 13.3 μM. The interaction of BD-105 with glucagon results in changes in fluorescence intensity, and it can colocalize with glucagon in cells and tissues. This selective cell stain specifically targets glucagon-secreting cells without staining insulin-secreting cells or non-endocrine control cells. BD-105 is suitable for glucagon imaging in live cells and tissues.

BD-105 (500 nM; 24 h) specifically labels glucagon-secreting AlphaTC1 cells without detecting a staining signal in BetaTC6 or HeLa cells. In buffer solution, BD-105 (10 μM) exhibits a maximum absorption wavelength of 550 nm and a maximum emission wavelength of 568 nm, with a quantum yield of approximately 0.012. Upon the addition of 200 μM glucagon to 1 μM BD-105, the quantum yield increases to 0.151, while the maximum absorption and emission wavelengths shift to 553 nm and 570 nm, respectively.