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BCECF Acetoxymethyl ester is a fluorescent indicator used to measure intracellular pH levels. It is converted to BCECF by intracellular esterases. Intracellular pH measurement involves ratio imaging, which determines the pH-dependent ratio between emission intensities when the dye is excited at 490 nm (detected at 535 nm) and at 440 nm.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 10 mg | Inquiry | Inquiry | Inquiry | |
| 50 mg | Inquiry | Inquiry | Inquiry |
| Description | BCECF Acetoxymethyl ester is a fluorescent indicator used to measure intracellular pH levels. It is converted to BCECF by intracellular esterases. Intracellular pH measurement involves ratio imaging, which determines the pH-dependent ratio between emission intensities when the dye is excited at 490 nm (detected at 535 nm) and at 440 nm. |
| Molecular Weight | 880.76 |
| Formula | C42H40O21 |
| Cas No. | 1119449-23-8 |
| Smiles | O=C(OCOC(=O)C)C1=CC=C2C(=C1)C(=O)OC23C=4C=C(C(OCOC(=O)C)=CC4OC=5C=C(OCOC(=O)C)C(=CC53)CCC(=O)OCOC(=O)C)CCC(=O)OCOC(=O)C |
| Storage | Store at -20°C Shipping with blue ice/Shipping at ambient temperature. |
Dissolve 2 mg of the compound in 100 μL DMSO
to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO
stock solution to 400 μL PEG300
and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O
and mix thoroughly until a homogeneous solution is obtained.
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