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Anti-TUBB3 Antibody (1C239)
😃Good
Catalog No. TMAB-01918
Alias Tubulin beta-III, Tubulin beta-4 chain, Tubulin beta-3 chain, TUBB4, TUBB3
Anti-TUBB3 Antibody (1C239) is a Mouse antibody targeting TUBB3. Anti-TUBB3 Antibody (1C239) can be used in WB,IHC-P,IHC-Fr,IF.
Anti-TUBB3 Antibody (1C239)
Anti-TUBB3 Antibody (1C239)
😃Good
Catalog No. TMAB-01918Alias Tubulin beta-III, Tubulin beta-4 chain, Tubulin beta-3 chain, TUBB4, TUBB3
Anti-TUBB3 Antibody (1C239) is a Mouse antibody targeting TUBB3. Anti-TUBB3 Antibody (1C239) can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $527 | 7-10 days | |
| 500 μL | $754 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-TUBB3 Antibody (1C239) is a Mouse antibody targeting TUBB3. Anti-TUBB3 Antibody (1C239) can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | Tubulin beta-III, Tubulin beta-4 chain, Tubulin beta-3 chain, TUBB4, TUBB3 |
| Ig Type | IgG |
| Clone | 1C239 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (TUBB3 (Neuronal Marker)) Monoclonal Antibody, Unconjugated (TMAB-01918) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (TUBB3 (Neuronal Marker)) Monoclonal Antibody, Unconjugated (TMAB-01918) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (TUBB3 (Neuronal Marker)) Monoclonal Antibody, Unconjugated (TMAB-01918) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (human cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (TUBB3 (Neuronal Marker)) Monoclonal Antibody, Unconjugated (TMAB-01918) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded (Human left parietal lobe); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (TUBB3 (Neuronal Marker)) Monoclonal Antibody, Unconjugated (TMAB-01918) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TUBB3 (Neuronal Marker) Monoclonal Antibody, Unconjugated (TMAB-01918) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nucleus. 7. 25 μg total protein per Lane of various lysates probed with Tubb3 monoclonal antibody, unconjugated (TMAB-01918) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. 8. 25 μg total protein per Lane of various lysates probed with TUBB3 monoclonal antibody, unconjugated (TMAB-01918) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-5000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Cytoplasm, cytoskeleton. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Neuronal Marker Beta III tubulin is abundant in the central and peripheral nervous systems (CNS and PNS) where it is prominently expressed during fetal and postnatal development. As exemplified in cerebellar and sympathoadrenal neurogenesis, the distribution of beta III is neuron-associated, exhibiting distinct temporospatial gradients according to the regional neuroepithelia of origin. However, transient expression of this protein is also present in the subventricular zones of the CNS comprising putative neuronal- and/or glial precursor cells, as well as in Kulchitsky neuroendocrine cells of the fetal respiratory epithelium. This temporally restricted, potentially non-neuronal expression may have implications in the identification of presumptive neurons derived from embryonic stem cells. |
| Immunogen | KLH conjugated synthetic peptide: human TUBB3 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Neuronal,Tubulin,Microtubules |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Keywords
Tubulinβ-IIITubulin-βIIITubulinβIIITubulinβ-4 chainTubulinβ4 chainTubulinβ-3 chainTubulinβ3 chainTubulin β-IIITubulin β-4 chainTubulin β4 chainTubulin β-3 chainTubulin β3 chainTubulin b-IIITubulin bIIITubulin betaIIITubulin beta4 chainTubulin beta3 chainTubulin b-4 chainTubulin b4 chainTubulin b-3 chainTubulin b3 chainTUBB 4TUBB 3
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