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Anti-TUBB3 Antibody (1C239)

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Catalog No. TMAB-01918
Alias Tubulin beta-III, Tubulin beta-4 chain, Tubulin beta-3 chain, TUBB4, TUBB3

Anti-TUBB3 Antibody (1C239) is a Mouse antibody targeting TUBB3. Anti-TUBB3 Antibody (1C239) can be used in WB,IHC-P,IHC-Fr,IF.

Anti-TUBB3 Antibody (1C239)

Anti-TUBB3 Antibody (1C239)

😃Good
Catalog No. TMAB-01918Alias Tubulin beta-III, Tubulin beta-4 chain, Tubulin beta-3 chain, TUBB4, TUBB3
Anti-TUBB3 Antibody (1C239) is a Mouse antibody targeting TUBB3. Anti-TUBB3 Antibody (1C239) can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3737-10 days
200 μL$5277-10 days
500 μL$7547-10 days
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Product Introduction

Bioactivity
Description
Anti-TUBB3 Antibody (1C239) is a Mouse antibody targeting TUBB3. Anti-TUBB3 Antibody (1C239) can be used in WB,IHC-P,IHC-Fr,IF.
AliasTubulin beta-III, Tubulin beta-4 chain, Tubulin beta-3 chain, TUBB4, TUBB3
Ig Type
IgG
Clone
1C239
Reactivity
Human,Mouse,Rat
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (TUBB3 (Neuronal Marker)) Monoclonal Antibody, Unconjugated (TMAB-01918) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (TUBB3 (Neuronal Marker)) Monoclonal Antibody, Unconjugated (TMAB-01918) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (TUBB3 (Neuronal Marker)) Monoclonal Antibody, Unconjugated (TMAB-01918) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (human cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (TUBB3 (Neuronal Marker)) Monoclonal Antibody, Unconjugated (TMAB-01918) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (Human left parietal lobe); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (TUBB3 (Neuronal Marker)) Monoclonal Antibody, Unconjugated (TMAB-01918) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TUBB3 (Neuronal Marker) Monoclonal Antibody, Unconjugated (TMAB-01918) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nucleus.
7. 25 μg total protein per Lane of various lysates probed with Tubb3 monoclonal antibody, unconjugated (TMAB-01918) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
8. 25 μg total protein per Lane of various lysates probed with TUBB3 monoclonal antibody, unconjugated (TMAB-01918) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-5000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Monoclonal
Host SpeciesMouse
Subcellular LocalizationCytoplasm, cytoskeleton.
ConstructionHybridoma Monoclonal Antibody
PurificationProtein G purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundNeuronal Marker Beta III tubulin is abundant in the central and peripheral nervous systems (CNS and PNS) where it is prominently expressed during fetal and postnatal development. As exemplified in cerebellar and sympathoadrenal neurogenesis, the distribution of beta III is neuron-associated, exhibiting distinct temporospatial gradients according to the regional neuroepithelia of origin. However, transient expression of this protein is also present in the subventricular zones of the CNS comprising putative neuronal- and/or glial precursor cells, as well as in Kulchitsky neuroendocrine cells of the fetal respiratory epithelium. This temporally restricted, potentially non-neuronal expression may have implications in the identification of presumptive neurons derived from embryonic stem cells.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human TUBB3
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Neuronal,Tubulin,Microtubules
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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